Objective To screen the chemical compounds in Qinzhu Liangxue Mixture that have therapeutic effects on psoriasis.Methods The compounds in the Qinzhu Liangxue Mixture were isolated and purified by the chromatography technology,the compound structures were identified by the spectral data,and the in vitro anti-psoriasis activity was studied by the MTT assay.Results Eleven compounds were isolated and identified from Qinzhu Liangxue Mixture,including liquiritigenin(compound 1),liquiritin(compound 2),liquiritin apioside(compound 3),cimifugin(compound 4),4'-O-β-D-glucosyl-5-O-methylvisamminol(compound 5),baicalin(compound 6),baicalein(compound 7),wogonin(compound 8),paeonol(compound 9),benzoic acid(compound 10),and ethylparaben(compound 11).Compounds 2-3 and 5-7 showed different degrees of inhibitory effects on the proliferation of keratinocytes,and compound 6 had the strongest inhibitory activity on the proliferation of keratinocytes,with a half maximal inhibitory concentration(IC50)of 2.5 μmol/L.Conclusion Compounds 1-11 were isolated for the first time from Qinzhu Liangxue Mixture and have anti-proliferative effects on keratinocytes.
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