HPLC Fingerprints and Chemical Pattern Recognition of Changwei Powder
Objective To provide a reference for the quality evaluation of Changwei Powder.Methods The high-performance liquid chromatography(HPLC)fingerprint was established,the chromatographic column was Agilent Eclipse XDB-C18 column(250 mm × 4.6 mm,5 µm),the mobile phase was acetonitrile-0.2％phosphoric acid solution(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 226 nm,the column temperature was 25 ℃,and the injection volume was 10 μL.The Similarity Evaluation System for Fingerprint Chromatographic of Traditional Chinese Medicine(Version 2012)was used to establish HPLC overlay fingerprints of 10 batches of samples and conduct similarity evaluation.The applicability of methods for overall similarity and improved extent similarity was investigated,and the quality was comprehensively evaluated by combining clustering analysis and principal component analysis.Results A total of 18 common peaks were identified in the HPLC fingerprints,and peaks 1,7,9,11,14,and 16 were determined to be hesperidin,cinnamic acid,cinnamaldehyde,eugenol,evodiamine,and rutaecarpine,respectively.The similarity of fingerprints,overall similarity,and improved extent similarity were 0.996-1.000,0.886-0.955,and 0.878-0.951,respectively.According to the overall similarity and improved extent similarity,samples numbered S3-S4,S6-S7,and S9 were grouped into one category,while samples with other numbers were grouped into another category.Cluster analysis was used to group 10 batches of samples into three categories:samples numbered S3-S4,S6-S7 and S9,samples numbered S8 and S10,and samples numbered S1-S2 and S5.According to principal component analysis,the cumulative variance contribution rate of the four principal components was 90.662％.The comprehensive scores of the principal components were ranked in descending order of sample number as S8＞S9＞S6＞S3＞S7＞S10＞S4＞S5＞S1＞S2.Conclusion The established method is simple,fast,and highly feasible,which can be used to comprehensively evaluate the quality of Changwei Powder.
万方数据
维普
