首页|麻黄汤对模型大鼠鼻黏膜免疫屏障功能的影响

麻黄汤对模型大鼠鼻黏膜免疫屏障功能的影响

Effect of Mahuang Decoction on the Immune Barrier Function of Nasal Mucosa in Model Rats

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目的 探讨麻黄汤对模型大鼠鼻黏膜屏障功能的影响及作用机制.方法 以冷-热循环刺激复制鼻黏膜免疫功能低下大鼠模型,并予麻黄汤灌胃,取鼻黏膜进行转录组学测序,并用qPCR和Western blot法验证结果.结果 给予模型大鼠麻黄汤后,可影响β-干扰素水平及细胞外基质、细胞因子应答等进程,并通过影响肿瘤坏死因子信号通路、JAK-STAT信号通路等发挥作用.麻黄汤通过激活IL-22/STAT3 通路上调LCN2、S100A8、MUC5b、MMP13、CXCL10、CCL20、IL17RA差异基因的表达及IL-22、STAT3、p-STAT3、p-STAT1 的蛋白表达,并降低TRAF6 的mRNA表达.结论 麻黄汤可通过IL-22/STAT3 信号通路提高模型大鼠鼻腔局部免疫屏障功能.
Objective To investigate the effect of Mahuang Decoction on the nasal mucosal barrier function in model rats and relevant mechanism.Methods Rat models of nasal mucosal immune dysfunction were constructed by cold-hot cycle stimulation and were given Mahuang Decoction by gavage.The nasal mucosa was taken for transcriptome sequencing,and the qPCR and Western blot were used for verification test.Results Mahuang Decoction could affect the β-interferon level,extracellular matrix,response to cytokines and other processes in model rats,and it also played a role by affecting the tumor necrosis factor signaling pathway,JAK-STAT signaling pathway,etc.Mahuang Decoction upregulated the expression of differential genes including LCN2,S100A8,MUC5b,MMP13,CXCL10,CCL20,IL17RA and the protein expression of IL-22,STAT3,p-STAT3 and p-STAT1,and decreased the mRNA expression of TRAF6 by activating the IL-22/STAT3 pathway.Conclusion Mahuang Decoction can improve the local immune barrier function in the nasal cavity of model rats through the IL-22/STAT3 signaling pathway.

Mahuang Decoctionratnasal mucosainterleukin-22signal transducer and activator of transcription 3immune barriertranscriptomics

朱紫陌、崔白梅、杨居崩、赵显芳、李秀芳

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云南中医药大学中药学院,云南 昆明 650500

云南省红河哈尼族彝族自治州个旧市中西医结合医院,云南 红河 661000

麻黄汤 大鼠 鼻黏膜 白细胞介素22 信号转导和转录激活因子3 免疫屏障 转录组学

国家自然科学基金云南省科技厅科技计划云南省科技厅科技计划

81860724202001AZ070001-001202305AC160043

2024

10.3969/j.issn.1006-4931.2024.20.009

中国药业
重庆市食品药品监督管理局

中国药业

CSTPCD
影响因子：1.369
ISSN：1006-4931
年,卷(期)：2024.33(20)