Optimization of Purification Process of Phenolic Acids and Flavonoids in Xuanfu Daizhe Decoction by Principal Component Analysis Combined with Box-Behnken Design-Response Surface Methodology
Objective To optimize the separation and purification process of phenolic acids and flavonoids in Xuanfu Daizhe Decoction.Methods The Box-Behnken design-response surface methodology(BBD-RSM)was used to design the test with elution flow rate,ethanol elution volume fraction,and ethanol elution volume as the investigation factors.The HPD400 macroporous resin was used to separate and purify six phenolic acids and three flavonoids in Xuanfu Daizhe Decoction.The ultra-high-performance liquid chromatography(UPLC)method was used to determine the components in the eluent.The ultraviolet-visible spectrophotometry(UV-Vis)was used to determine the mass fraction of total flavonoids in the eluent.The principal component analysis combined with BBD-RSM was used to evaluate the model,predict the optimal purification process,and the verification test was carried out.Results The optimal purification process was as follows:the flow rate was 2.6 mL/min,the ethanol elution volume fraction was 53%,and the ethanol elution volume was 2.4 BV.After verification,the mass fraction of total flavonoids under the optimal purification process was(69.33±1.66)%;the contents of chlorogenic acid,caffeic acid,isoquercitrin,isochlorogenic acid B,isochlorogenic acid A,1,5-dicaffequinic acid,isorhamnoside,isochlorogenic acid C,and quercetin were(2.67±0.04)μg/mg,(15.34±0.27)μg/mg,(12.36±0.17)μg/mg,(16.01±0.22)μg/mg,(18.25±0.22)μg/mg,(96.24±1.44)μg/mg,(6.85±0.07)μg/mg,(22.55±0.35)μg/mg,(5.78±0.07)μg/mg respectively.Conclusion The optimal purification process of phenolic acids and flavonoids in Xuanfu Daizhe Decoction by macroporous adsorption resin is stable and feasible,which can provide a reference for further pharmacological research.
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维普
