Distribution Characteristics of Qingre Liangxue Formula in Rats
Objective To investigate the distribution characteristics of Qingre Liangxue Formula in rats.Methods The ultra-high-performance liquid chromatography-quadrupole/electrostatic field orbitaltrap high-resolution mass spectrometry(UPLC-Q-Exactive Orbitrap MS)was adopted.The chromatographic column was the Thermo Accucore aQ C18 column(150 mm×2.1 mm,2.6 μm),the mobile phase was methanol-0.1%formic acid aqueous solution(gradient elution),the flow rate was 0.3 mL/min,the column temperature was 35℃,and the injection volume was 3 μL.The heating electrospray ionization(HESI)was adopted,the spray voltage was 3.5 kV,the sheath gas(N2)flow rate was 40 arb,the auxiliary gas(N2)flow rate was 10 arb,the auxiliary heater temperature was 350℃,and the capillary temperature was 320℃.The Full MS/dd-MS2 mode with positive and negative ions was adopted,with a scanning range of m/z 100-1 500 and collision energy of 30 eV.The rats were given Qingre Liangxue Formula(93.6 g/kg)by gavage once a day for 4 d.The plasma,different tissues and excreta were collected at different time points after administration to identify the distribution characteristics of prototype components.Results A total of 18 prototype components in vivo and 22 prototype components in excreta were identified.Ten,four,six,seven prototype components of the Qingre Liangxue Formula were detected in rat plasma samples at 0.5 h,1 h,1.5 h and 2 h after the last administration,respectively.Nine,seven,seven,five,seven,nine prototype components were detected in the heart,liver,spleen,lung,kidney,brain tissues at 2 h after the last administration,respectively.Eleven and fifteen prototype components were detected in the feces and urine from 8 h after administration on the 3rd day to before administration on the 4th day,respectively.Conclusion The components in vivo of Qingre Liangxue Formula are comprehensively identified and analyzed in this study,and the study can lay a foundation for the subsequent quality evaluation and studies on pharmacological substances and mechanisms of Qingre Liangxue Formula.
UPLC-Q-Exactive Orbitrap MSQingre Liangxue Formulaprototype componentin vivo distributionpharmacological substance
万方数据
维普
