Objective To establish a method for identifying the Galli Gigerii Endothelium Corneum and its adulterants,and to study the quality of the medicinal material.Methods The ultra-high-performance liquid chromatography(UPLC)method was adopted;the chromatographic column was the Agilent Zorbax SB C18 column(100 mm×2.1 mm,1.8 μm),the mobile phase was acetonitrile-water(4∶1,V/V)-0.1%phosphoric acid aqueous solution(gradient elution),the flow rate was 0.3 mL/min,the detection wavelength was 260 nm,the column temperature was 30℃,and the injection volume was 2 μL.The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine(version 2012)was used to plot the chromatogram of 29 batches of samples(18 certified products and 11 adulterants),the common peaks were marked and identified.The SIMCA-P 14.0 software was used for cluster analysis,the partial least squares-discriminant analysis(PLS-DA)and principal component analysis(PCA)were used for principal component analysis and clustering.The UPLC method was used to determine the contents of identified components,and the characteristic chromatogram of the main components of Gallus gallus domesticus feed was compared with that of Galli Gigerii Endothelium Corneum to analyze the sources of flavonoids.Results There were nine common peaks in the characteristic chromatogram of 18 batches of Galli Gigerii Endothelium Corneum,and four flavonoids were identified,including genistin,daidzein,glycitein and genistein.Galli Gigerii Endothelium Corneum and its adulterants can be distinguished by the cluster analysis,PLS-DA method and PCA method.There were common(characteristic)peaks in the chromatograms of main components of Gallus gallus domesticus feed and Galli Gigerii Endothelium Corneum.Conclusion The established method can effectively identify the Galli Gigerii Endothelium Corneum and its adulterants.It is preliminary inferred that the flavonoids in the Galli Gigerii Endothelium Corneum are induced by long-term enrichment of Gallus gallus domesticus feed.
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