Optimization of Extraction Process of Xiaoyu Gel Plaster by Orthogonal Test and Principal Component Analysis
Objective To optimize the extraction process of Xiaoyu Gel Plaster.Methods The volatile oil extraction process was optimized by the L9(34)orthogonal test with the volatile oil content as the evaluation indicator,and with the water addition amount,soaking time and extraction time as the influencing factors.Then,the verification test was carried out.The non-volatile component extraction process was optimized by the L9(34)orthogonal test with the dry extract yield,asiaticoside,luteolin and baicalin contents as the evaluation indicators,with the comprehensive score of principal component as the comprehensive indicator,and with the volume fraction of ethanol,solvent dosage,and extraction frequency as the evaluation factors.Then,the verification test was carried out.Results The optimal extraction processes of volatile oil were as follows:adding 10 times the amount of water,soaking for 1.0 h,and extracting for 6 h;the volatile oil content of 25 g medicinal herbs(Schizonepetae Herba,Olibanum,Myrrha,Angelicae Sinensis Radix)was 1.6 mL.The optimal extraction processes of non-volatile components were as follows:using 10 times the amount of 60%ethanol as the solvent,extracting for three times,each time for 1 h;the average comprehensive score was 125.36 points in the verification test,with an RSD of 0.49%(n=3).Conclusion The optimal process is stable and feasible,which can retain potential active components at the greatest extent,and lay the foundation for new drug development.
Xiaoyu Gel Plasterorthogonal testvolatile componentnon-volatile componentextraction processprocess optimizationprincipal component analysis
万方数据
维普
