HPLC Fingerprint of Wulin Pills and Determination of Seven Components
Objective To provide a basis for the quality control of Wulin Pills.Methods High-performance liquid chromatography(HPLC)method was used to establish the fingerprint of six batches of Wulin Pills.The chromatographic column was the Anpel Athena C18 column(250 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-0.1%phosphoric acid aqueous solution(gradient elution),the flow rate was 1.0 mL/min,the detection wavelengths were 240 nm(fingerprint establishment and determination of geniposide,paeoniflorin,ammonium glycyrrhizinate)and 327 nm(determination of chlorogenic acid,palmatine hydrochloride,berberine hydrochloride,ligustilide),the column temperature was 25℃,and the injection volume was 10 μL.The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine(version 2012)was used for similarity evaluation,the common peaks were marked and attributed,the components were identified and determined.Results The similarity between the fingerprint of the six batches of samples and the reference fingerprint was in the range of 0.992-1.000.Twelve common peaks were marked and attributed to medicinal herbs,and seven components were identified.The linear ranges of geniposide,paeoniflorin,ammonium glycyrrhizinate,chlorogenic acid,palmatine hydrochloride,berberine hydrochloride and ligustilide were 2.77-27.74 μg/mL,16.10-161.04 μg/mL,8.54-85.42 μg/mL,0.71-7.05 μg/mL,2.75-27.54 μg/mL,3.14-31.35 μg/mL,5.43-54.27 μg/mL(R2≥0.999 5,n=6)respectively.The RSDs of precision,stability and repeatability tests were all lower than 2.0%.The average recovery rates of the above seven components were 93.48%,103.15%,104.68%,93.53%,95.76%,104.85%,97.25%,with RSDs of 2.50%,1.90%,1.67%,2.05%,1.99%,1.48%,2.33%(n=6)respectively.Conclusion The established method in this study can be used to establish the HPLC fingerprint of Wulin Pills and determine the contents of seven components,which provides a basis for more comprehensive quality control of Wulin Pills.
万方数据
维普
