Determination of Hydroxysafflor Yellow A in Shiguogong Medicinal Liquor by HPLC
Objective To establish a high-performance liquid chromatography(HPLC)method for determining the content of hydroxysafflor yellow A in Shiguogong Medicinal Liquor.Methods The chromatographic column was the Kromasil 100-5-C18 column(250 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-0.1%phosphoric acid aqueous solution(12∶88,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 403 nm,the column temperature was 30℃,and the injection volume was 20 μL.Results The linear range of hydroxysafflor yellow A was 2.814-45.025 μg/mL(r=0.999 5,n=5).The RSDs of precision,stability and repeatability tests were all lower than 2.0%.The average recovery rate of hydroxysafflor yellow A was 102.12%with an RSD of 2.44%(n=6).Conclusion The established method is easy,accurate and stable,which simplifies the composition of mobile phase and the preparation of test solution,and it can be used for the quality control of Shiguogong Medicinal Liquor.
万方数据
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