Changes in the Content of Magnolol and Honokiol During the"Fahan"Process of Magnolia Officinalis from Different Source Origins or with Different Tree Ages
Objective To investigate the changing trend of of magnolol and honokiol content during the"Fahan"process of Magnolia officinalis.Methods High-performance liquid chromatography(HPLC)method was used for the content determination of magnolol and honokiol during the"Fahan"process of 20-year-old Magnolia officinalis from Pengzhou and 20-year-old and 40-year-old Magnolia officinalis from Ya'an.The chromatographic column was Wondasil C18 column(150 mm × 4.6 mm,5 μm),the mobile phase was methanol water(80:20,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 294 nm,the column temperature was 30 ℃,and the injection volume was 10 μL.Results The linear ranges of magnolol and honokiol were 0.006 1-0.194 0 mg/mL and 0.001 8-0.056 0 mg/mL(r 0.999 8,n=6),respectively.The limits of detection were 0.000 97 mg/mL and 0.002 24 mg/mL,respectively,and the limits of quantification were 0.012 10 mg/mL and 0.003 50 mg/mL,respectively.The RSDs of precision,stability,and repeatability test results were all lower than 2.0％.The average recoveries of magnolol and honokiol were 98.79％and 99.29％,with RSDs of 1.71％and 1.20％(n=6),respectively.The content of magnolol and honokiol in 20-year-old Magnolia officinalis from Pengzhou and Ya'an reached its peak value on the 7th day of"Fahan"(3.48％and 3.83％,respectively).The content of magnolol and magnolol in 40-year-old Magnolia officinalis from Ya'an reached its peak value on the 5th day(12.14％).Conclusion The established method is quick,efficient,accurate,and highly specific,which can be used for the content determination of magnolol and honokiol in Magnolia officinalis.The age of trees is the main influencing factor of the changes in magnolol and honokiol in Magnolia officinalis during the"Fahan"process.
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