Simultaneous Determination of Seven Anti-Inflammatory Active Components in Huangge Peiyuan Ointment by QAMS
Objective To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous determination of seven anti-inflammatory active components(morroniside,loganin,prim-O-glucosylcimifugin,galycosin 7-O-β-D-glucopyranoside,5-O-methylvisammioside,icariin,schisandrin)in Huangge Peiyuan Ointment.Methods Huangge Peiyuan Ointment was extracted with 50％methanol.The chromatographic column was Agilent Eclipse XDB-C18 column(250 mm × 4.6 mm,5 µm),the mobile phase was acetonitrile-0.2％phosphoric acid aqueous solution(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 260 nm,the column temperature was 30 ℃,and the injection volume was 5 μL.With icariin as the internal standard,the relative correction factors of the other six components were calculated,and their contents were determined by the QAMS method and the external standard method.The reliability of the results was verified through the relative errors of the two methods.Results The linear ranges of morroniside,loganin,prim-O-glucosylcimifugin,galycosin 7-O-β-D-glucopyranoside,5-O-methylvisammioside,icariin,and schisandrin were 14.21-454.71 μg/mL,6.65-212.89 μg/mL,1.36-43.54 μg/mL,1.18-37.64 μg/mL,1.64-52.42 µg/mL,3.38-108.11 μg/mL,and 0.57-18.17 μg/mL(r＞0.999 5,n=6),respectively.The RSDs of precision,stability,and repeatability test results were all lower than 3.0％(n=6).The average recoveries of the above seven components were in the ranges of 95.68％-99.50％,with RSDs of 0.55％-2.41％(n=6).With icariin as the internal standard,the relative correction factors of morroniside,loganin,prim-O-glucosylcimifugin,galycosin 7-O-β-D-glucopyranoside,5-O-methylvisammioside,icariin,and schisandrin were 2.995 5,3.947 6,2.225 1,0.584 3,2.154 9,and 1.255 7,respectively.The relative deviation between the content determination result of the QAMS method and that of the external standard method was ranged from-1.3％to 1.9％.Conclusion The method is simple,reliable,and accurate,which can be used for the content determination of seven anti-inflammatory active components in Huangge Peiyuan Ointment.
Huangge Peiyuan OintmentQAMSrelative correction factorsactive componentsanti-inflammatoryquality control
万方数据
维普
