Study on HPLC Fingerprint and Chemical Pattern Recognition of Ginseng Folium Formula Granules
Objective To provide a reference for the quality control and evaluation of Ginseng Folium Formula Granules.Methods The high-performance liquid chromatography(HPLC)method was adopted;the chromatographic column was the CORTECS T3 column(150 mm × 4.6 mm,2.7 μm),the mobile phase was acetonitrile-0.1％phosphoric acid aqueous solution(gradient elution),the flow rate was l.0 mL/min,the detection wavelength was 203 nm,the column temperature was 25 ℃,and the injection volume was 10 μL.The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Version)was used to establish HPLC fingerprint of 21 batches of samples,the common peaks were marked,and the similarity evaluation was performed.The quality of samples from different batches was evaluated by the cluster analysis(CA),principal component analysis(PCA),and orthogonal partial least squares-discriminant analysis(OPLS-DA)methods,and the markers were screened.Results A total of 14 common peaks were marked,of which four were identified,including kaempferol-3-O-sophoroside,ginsenoside Rg1,ginsenoside Re and ginsenoside Rd.The similarity of 21 batches of samples was greater than 0.900.The CA showed that 21 batches of samples were clustered into three groups.The PC A showed that four principal components were extracted,with a cumulative variance contribution rate of 91.119％.The OPLS-DA showed that kaempferol-3-O-sophoroside had a significant effect on Ginseng Folium Formula Granules.Conclusion This method is simple,feasible,repeatable,precise and stable,which can provide a reference for quality control and overall evaluation of Ginseng Folium Formula Granules.
万方数据
维普
