Simultaneous Determination of Two Components in Bawei Shihuihua Pills by HPLC
Objective To establish a high-performance liquid chromatography(HPLC)method for simultaneous determination of ellagic acid(EA)and hydroxysafflor yellow A(HYSA)in Bawei Shihuihua Pills.Methods The chromatographic column was the ZORBAX Eclipse Plus C18 column(250 mm × 4.6 mm,5 μm),the mobile phase was 0.1％phosphoric acid aqueous solution-methanol(gradient elution),the flow rate was 1.0 mL/min,the detection wavelengths were 253 nm(EA)and 403 nm(HYSA),the column temperature was 35℃,and the injection volume was 10 μL.Results The linear ranges of EA and HYSA were 0.73-14.52 μg/mL,3.97-79.45 μg/mL(r=0.999 8,1.000 0,n=7)respectively.The limits of detection of EA and HYSA were 0.29 μg/mL,1.59 μg/mL,the limits of quantification were 0.58 μg/mL,3.18 μg/mL respectively.The RSDs of precision,stability and repeatability tests were all lower than 2.0％.The average recovery rates of EA and HYSA were 103.36％,102.57％with RSDs of 3.00％,2.75％(n=6)respectively.Conclusion This method is easy and accurate,which can be used to simultaneously determine the contents of EA and HYSA in Bawei Shihuihua Pills.
万方数据
维普
