Determination and Analysis of 16 Trace Elements in Tibetan Medicine Hypecoum Leptocarpum
Objective To provide a reference for the determination of trace element and standardized cultivation of Hypecoum leptocarpum.Methods The inductively coupled plasma-optical emission spectrometry(ICP-OES)method was used to determine the contents of aluminum(Al),barium(Ba),calcium(Ca),potassium(K),magnesium(Mg),manganese(Mn),sodium(Na),phosphorus(P),strontium(Sr),titanium(Ti),boron(B),iron(Fe),lithium(Li),vanadium(V)and zinc(Zn)in 27 batches of samples from different producing areas in Qinghai Province;the plasma radio frequency power was 1.20 kW,the plasma gas flow rate was 15 L/min,the atomizer flow rate was 0.75 L/min,the auxiliary air flow rate was 1.5 L/min,the observation time was 10 min,the single reading time was 3 s,the stabilization time was 15 s,the plasma gas,atomization gas and auxiliary gas were all high-purity argon,the detection wavelengths were 396.2,455.4,317.9,766.5,279.6,257.6,589.6,213.6,407.8,336.1,249.8,238.2,670.8,292.4,213.9 nm respectively.The atomic fluorescence spectrometry was used to determine the content of selenium(Se),the lamp current was 100 mA,the shielding gas flow rate was 1 000 mL/min,the negative high voltage was 340 V,the carrier gas flow rate was 500 mL/min,the atomizer height was 8 mm,the injection volume the 2 mL,the carrier gas and shielding gas were both high-purity argon.Principal component analysis(PCA)and discriminant correspondence analysis(DCA)were used to determine the content of various trace elements.Results The linear ranges of 15 elements and Se were 0.02-10 μg/mL and 0-40 μg/L respectively(r＞0.999 6).The limits of detection(LOD)were in the range of 0.018 to 3.000 mg/L,0.002 mg/L respectively.The RSDs of precision and repeatability tests were both lower than 3.0％.The average recovery rates of 15 elements and Se were in the range of 94.01％to 102.15％,91.44％respectively,with the RSDs lower than 10％(n=6).The average content of 16 elements in each batch of samples was ranked as K＞Ca＞Mg＞P＞Na＞Fe＞Al＞Sr＞Zn＞Mn＞Ba＞B＞Ti＞V＞Li＞Se.The PCA showed that Al,Fe,Li and Mn were the characteristic trace elements of Tibetan medicine Hypecoum leptocarpum.The DCA showed that Fe,Al,Ca,Ti,Mn,V,Ba contents were positively correlated with the altitude and negatively correlated with the longitude and latitude;while K,Zn,Sr,Mg,Na,P contents were the opposite;and Se,Li,B contents were not correlated with the altitude or longitude and latitude.Conclusion The established method is easy,stable and reliable,which can be used for the determination of trace element in Hypecoum leptocarpum.The content of various trace elements in Hypecoum leptocarpum from different producing areas in Qinghai Province varies greatly,and has a certain correlation with the altitude,longitude and latitude of the producing areas.
Hypecoum leptocarpumtrace elementcontent determinationaltitudelongitudelatitudeQinghai Province
万方数据
维普
