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高效液相法测定视力健咀嚼片中原花青素的含量

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目的:采用高效液相色谱法对视力健咀嚼片中原花青素的含量进行了测定.方法:采用高效液相法,色谱柱:迪马公司产Diamonsil C18柱(4.6mm×200mm,5μm),柱温:25℃,流动相:V( 乙腈):V(水)=8:92,检测波长:280nm,进样量:10μL,流速:1ml/min.结果:原花青素在(0.32~2.56)mg/mL范围内具有良好的线性关系,回收率为98.71%,RSD=0.76%(n=6).结论:本方法简便、快速、准确、可靠,可用于视力健咀嚼片中原花青素的含量测定的方法.
Determination of Proanthocyanidins in Shilijian Chewable Tablets by HPLC
Objective:The content of oanthocyanidins in shilijian chewable tablets was determined by HPLC.Methods:The determination was carried out with C18 column (5 μm; 150mm × 4.6mm) with the mobile phase of 8% acetonitrile and 92% water. The column temperature, flow rate and detection wavelength were 25℃, 1 ml/min and 280 nm respectively. Results:There was a good linear relationship between absorption and proanthocyanidins content within the range of (0.32~2.56) mg/mL. Recovery rate and RSD of the method were of 98.71 % and 0.76%.Conclusion:This method is simple, fast, accurate and reliable to be suitable for etermination of proanthocyanidins content in Shilijian Chewable Tablets.

ProanthocyanidinsContent determinationHPLC

王建明、李早慧

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黑龙江中医药大学中医药研究院,哈尔滨,150040

原花青素 含量测定 高效液相色谱法

2012

中国医药导刊
国家食品药品监督管理局信息中心

中国医药导刊

CSTPCD
影响因子:1.408
ISSN:1009-0959
年,卷(期):2012.14(1)
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