首页|南药巴戟天对大鼠运动行为及骨骼肌结构与功能的影响

南药巴戟天对大鼠运动行为及骨骼肌结构与功能的影响

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目的:探究南药巴戟天对大鼠运动能力的影响及其作用的生理机制.方法:健康成年SD大鼠随机分为巴戟天组(n=8)及对照组(n=6).巴戟天组采用自然进食方式定量饲喂含巴戟天的饲料,对照组饲料中不含巴戟天.于给药前(W0)和给药4周(W4)、8周(W8)后作行为学检测,包括基础运动行为(步态实验)和应激运动行为(悬挂和摇杆实验);行为学测试结束后,在体制备坐骨神经-腓肠肌标本,于一侧坐骨神经施以单个电刺激,另一侧坐骨神经施以连续电刺激并描记疲劳曲线,分析巴戟天对骨骼肌收缩能力的影响;随后对腓肠肌作组织切片,探究巴戟天对肌纤维形态的影响.结果:与对照组大鼠行为学结果相比,巴戟天组大鼠的基础运动能力没有发生显著变化,但在应激状态下的运动能力明显增强;与对照组大鼠肌张力结果相比,巴戟天组大鼠由单刺激引起肌收缩的强度没有发生显著变化,但在连续电刺激描记的疲劳曲线上耐力明显增强;与对照组大鼠肌纤维形态相比,巴戟天组大鼠肌纤维横截面明显增大.结论:巴戟天增强大鼠在应激状态下的运动能力,可能与骨骼肌收缩耐力增强及肌纤维增粗有关.该研究结果为巴戟天的药理研究和产品应用开发提供科学指导.
Effects of Morinda officinalis on the Locomotor Behaviors,the Structures and Functions of Skeletal Muscles in Rats
Objective:To investigate the impact of Morinda officinalis on the locomotor capabilities of rats and to elucidate the associ-ated physiological mechanisms.Methods:Fourteen healthy adult Sprague-Dawley(SD)rats were randomly divided into the Morinda group(n=8)and the control group(n=6).The Morinda group was fed the diet containing Morinda officinalis by natural feeding method,while the diet of the control group did not contain Morinda officinalis.Behavioral assessments were conducted at baseline(W0),and after 4 weeks(W4)and 8 weeks(W8)of the feeding.The behavioral assessments included tests for basic motor behavior(gait analysis)and challenging motor tasks(suspension and shaking-rod tests).After the behavioral tests,sciatic nerve-gastrocnemius specimens were prepared in vivo,skeletal muscle contraction waves were recorded and analyzed following single electrical stimulation to one side of the sciatic nerve,and the fatigue curves were constructed from continuous electrical stimuli on the another side of the sciatic nerve to evaluate the effect of Morinda officinalis on the contractility of skeletal muscle.Then the gastrocnemiust issue was sectioned to examine the effects of Morinda officinalis on the morphology of muscle fibers.Results:Compared with the control group,there was no significant change in basic motor ability of rats in the Morinda group,but demonstrated significant increase in locomotor capabilities under the stress condi-tions.Muscle contractions in response to single stimulations did not differ significantly between the two groups,yet the fatigue curve recorded by continuous electrical stimulation showed a notable improvement in muscular endurance of rats in the Morinda group.Addi-tionally,the cross section of muscle fibers of rats in the Morinda group increased significantly compared to the control group.Conclu-sion:Morinda officinalis enhances locomotor abilities in rats under challenging conditions,which are potentially related to increased skel-etal muscular contraction endurance and thickening of muscle fibers.The results of this study offer a scientific basis for further pharmaco-logical research and the development of functional products based on Morinda officinalis.

Morinda officinalisLocomotor behaviorSkeletal muscleContraction tensionStress

段文艺、陈秋遐、庄颖茵、严红炜、黄宝怡、姚紫纯、张长征、周佩玲

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岭南师范学院教育科学学院,广东 湛江 524048

巴戟天 运动行为 骨骼肌 收缩张力 应激

广东省自然科学基金广东省普通高等学校自然科学研究重点项目广东省科技创新战略专项广东省科技创新战略专项广东省大学生创新创业训练计划岭南师范学院教育教学成果奖培育项目

2024A15150129132023ZDZX2036pdjh2023b0326pdjh2024a238S202310579016LSGJ2318

2024

中国医药导刊
国家食品药品监督管理局信息中心

中国医药导刊

影响因子：1.408
ISSN：1009-0959
年,卷(期)：2024.26(3)
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