Study on the Quality Control Method of Chufeng Yisun Granules
Objective:The quality control method of chufeng yisun granules was established by TLC and HPLC.Methods:TLC was used to qualitatively identify the components of Radix Paeoniae Rubra,Moutan Cortex,Angelicae Sinensis Radix,Chuanxiong Rhizoma,Rhei Radix et Rhizoma,Saposhnidoviae Radix,Ligustri Lucidi Fructus in chufeng yisun granules,and the content of baicalin in chufeng yisun granules was determined by HPLC.The Waters Atlantis dC18(4.6 mm×250 mm,5 µm)column was used with methanol-0.4%phosphate water(47∶53)as mobile phase.The detection wavelength was 280 nm.The flow rate was 1.0 mL·min-1.The column tem-perature was 25℃and the injection volume was 10 µL.Results:The qualitative identification methods of Radix Paeoniae Rubra,Mou-tan Cortex,Angelicae Sinensis Radix,Chuanxiong Rhizoma,Rhei Radix et Rhizoma,Saposhnidoviae Radix,Ligustri Lucidi Fructus in chufeng yisun granules were establised by TLC.The TLC chromatography showed the same color spots in the same position of the corre-sponding control drugs,and the negative samples showed no interference.A HPLC method for the determination of baicalin in chufeng yisun granules was established,which is highly specific,and baicalin has a good linear relationship in the range of 0.101~1.01 µg(r=0.999 7).The average recovery was 100.22%,and RSD was 0.84%.Conclusion:The TLC identification method and the HPLC method for content determination established in this study have strong specificity and good reproducibility,which can be used for the qual-ity control of chufeng yisun granules.