Content Determination of α-Cyperone and Cyperenone in Shugan Hewei Pills by HPLC-UV
Objective:To establish a content determination method of high performance liquid chromatography(HPLC-UV)for α-cyperone and cyperenone in shugan hewei pills,and to provide a basis for quality control of the content of vinegar-processed Cyperi Rhizoma in this preparation.Methods:The content of α-cyperone and cyperenone in shugan hewei pills were determined simultane-ously.The analysis was performed on a Agilent ZORBAX SB-C18 chromatography column(4.6 mm×250 mm,5 µm),with mobile phase consisting of methanol and water(65∶35)at the flow rate of 1.0 mL·min-1.The detection wavelength was 254 nm.The column temperature was 30℃,and the injection volume was 5 µL.Results:In the chromatogram of shugan hewei pills obtained by this method,α-cyperone and cyperenone have good separation effects from adjacent chromatographic peaks,meeting the requirements for content determination.The linear ranges of indicator components α-cyperone and cyperenone were 0.080 36-0.803 60 µg and 0.211 2-2.112 0 µg,respectively.The linear relationship within the range is good,and the correlation coefficients were all 1.000.The average spiked recov-ery rates were 102.0%(RSD:1.6%,n=6)and 102.4%(RSD:2.0%,n=6),respectively.The stability(24 h),repeatability and pre-cision were compliance with requirements.Conclusion:The established method to determin the content of α-cyperone and cyperenone by HPLC-UV is simple,fast and accurate,and can provide a basis for controlling the content of vinegar-processed Cyperi Rhizoma in shugan hewei pills.
万方数据
维普
