Effects of TROAP Gene Knockdown on the Proliferation and Migration of Cholangiocarcinoma Cell Lines
Objective:To investigate the effects of Trophinin-associated protein(TROAP)gene silencing on the proliferation and migration of human cholangiocarcinoma cell line RBE.Methods:RBE cells were cultured and transfected with negative control sequence and TROAP interference sequence in si-NC group and si-TROAP group respectively.Cell proliferation ability was detected by cell count-ing kit-8(CCK-8)and plate clone formation experiment.Apoptosis rate was detected by flow cytometry.Cell migration and invasion ability were detected by cell scratch test and Transwell method.The relative expressions of epithelial-mesenchymal transition(EMT)related proteins E-cadherin,N-cadherin,PCNA and apoptosis related proteins Bcl-2,Bax and caspase-3 in RBE cells were detected by Western bloting.Results:Compared with si-NC group,the cell proliferation rate and number of clones decreased,the cell apoptosis rate increased,and the cell migration and invasion ability decreased in the si-TROAP group(P<0.05).Compared with si-NC group,the relative expression levels of E-cadherin protein increased,and the relative expression levels of N-cadherin protein decreased in the si-TROAP group;the relative expression levels of Bax and caspase-3 increased,and the relative expression levels of Bcl-2 and PCNA pro-tein decreased in the si-TROAP group(P<0.05).Conclusion:TROAP gene is a potential oncogene in RBE cells.TROAP silencing can reduce the proliferation and migration ability of human cholangiocarcinoma cell line RBE by alleviating epithelial-mesenchymal transition.
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