Research of humoral immunity response of plague component vaccine in mice
Objective To evaluate the immune response of a new plague component vaccine,which composed of extracted F1 antigen and recombinant V antigen.Methods Mice were randomly divided into four experimental groups,and were tested at five weeks after the first immunization.The blood was collected from each animal by inner canthus.Bronchoalveolar lavage (BAL) was obtained by exposing the trachea and making a small incision.Through this cannula the lungs were repeatedly lavaged by slowly injecting and withdrawing phosphate buffered saline.Individual serum and BAL samples were examined for total anti-F1 and anti-rV IgG and IgA antibodies using ELISA.Results ELISA results showed that plague component vaccine could induce high titer of anti-F1 and anti-rV IgG in the serum,and low titer of anti-F1 IgA could be detected.Low titer of anti-F1 and anti-rV IgG rather than IgA could be detected in BAL.Conclusion The new plague vaccine can cause significant humoral immunity response in mice,which would be a new generation of plague vaccine.
PlagueAntibody formationComponent vaccineF1 antigenRecombinant V antigen
NETL
NSTL
万方数据
维普
