摘要
哺乳动物细胞表达系统具有强大的翻译后修饰功能,表达的重组蛋白更接近人源构象,因此被广泛用于治疗性重组蛋白的生产,其中应用最广泛的是中华仓鼠卵巢细胞.基因组学、转录组学、蛋白质组学以及代谢组学等研究的不断深入促进了哺乳动物细胞表达系统的不断完善.本文立足于哺乳动物细胞表达系统的研究现状,对表达载体的优化、宿主细胞和位点特异性重组等方面的最新进展进行综述.
Abstract
The mammalian cell expression system possesses a robust post-translational modification capability,resulting in recombinant proteins that closely resemble the human native conformation.As a result,it is extensively utilized for the production of therapeutic recombinant proteins,with Chinese hamster ovary cells being the most commonly employed cell line.Advances in genomics,transcriptomics,proteomics,and metabolomics have contributed to the enhancement of the mammalian cell expression system.This review provides an overview of the current research landscape surrounding the mammalian cell expression system,summarizing the latest advancements in optimizing expression vectors,host cells,and site-specific recombination techniques.
关键词
重组蛋白/哺乳动物细胞表达系统/中华仓鼠卵巢细胞/UCOE/位点特异性整合Key words
recombinant protein/mammalian cell expression system/Chinese hamster ovary cell/UCOE/site-specific integration引用本文复制引用
出版年
2024
NETL
NSTL
万方数据