Analysis of intestinal flora dysbiosis of mice with myocardial fibrosis induced by isoproterenol based on 16S rRNA sequencing
Objective To analyze intestinal flora dysbiosis in mice with isoproterenol-(ISO)-induced myocardial fibrosis(MF)based on 16S rRNA sequencing.Methods The MF mouse model was established by subcutaneous injection of low-dose ISO for 3 weeks.The cardiac systolic and ejection function of the mice were evaluated by ultrasound,myocardial injury and fibrosis were observed by HE and Masson staining,the expression of genes related to fibrosis and ventricular remodeling was detected by real-time fluorescent quantitative PCR,and the intestinal flora dysbiosis in MF mice were analyzed by 16S rRNA sequencing.Results The MF model was successfully established verified by ultrasonography,pathological staining and RT-qPCR.16S rRNA sequencing showed that the relative abundance of Firmicutes decreased and that of Bacteroides increased in the gut of MF mice.The relative abundance of Alistipes,Parabacteroide,Sphingomonas,Burkholderiales,Parasutterella and Sutterellaceae was significantly different between MF and control mice.Conclusion There exists intestinal flora dysbiosis in MF mice,and these different bacteria genera and their metabolites are involved in the occurrence and progression of MF.
万方数据
维普
