Differential gene expression of umbilical cord mesenchymal stem cells cultured by two-dimensional and three-dimensional
Objective To compare morphological and gene expression of two-dimensional(2D)and three-dimensional(3D)cultured umbilical cord mesenchymal stem cells(UC-MSCs).Methods The stably proliferating UC-MSCs were cultured using 3D culture with polyvinyl fluoride as a scaffold and traditional 2D culture methods,respectively.The inverted microscope and scanning electron microscope were used to observe and compare the differences in cell morphology and cell connection between 2D and 3D culture.Gene expression microarray technology was then used to detect the differentially expressed genes between 2D and 3D cultured cells,and bioinformatics analysis was performed using the KEGG database and GO database.Results Human UC-MSCs were successfully isolated and cultured,with CD105,CD73 and CD90 being highly expressed as shown by flow cytometry.UC-MSCs cultured in 3D were attached to the surface of the scaffold and grew outwards.Cell growth was observed in all layers of the scaffold,and cell-to-cell connections were tight.Microarray analysis of gene expression profiles showed that,compared with 2D UC-MSCs,the increased genes of 3D UC-MSCs were mainly related to biological processes,molecular functions,and cell components,and were involved in the regulation of multiple cell signaling pathways.Conclusion Compared with 2D culture,the 3D cell culture environment is more suitable for the growth of UC-MSCs.
万方数据
维普
