Objective To apply the risk matrix to assess the risk of nosocomial infection management in upper second-class general hospitals.Methods The incidence rate,infection site,increased length of hospital stay,increased hospitalization cost,outcome,and monthly incidence variation coefficient of patients with nosocomial infection in each department in 2021 were used to quantify the probability of occurrence and consequence indicators,and the risk identification of internal medicine,surger-y,and obstetrics and gynecology and children systems was carried out.Percentile method(P20,P40,P60,P80)was used to grade the occurrence possibility and consequence,establish the risk matrix table,and analyze and determine the high-risk depart-ments.Results The high-risk departments in internal medicine were oncology department,geriatric disease department,general medicine department and infectious disease department;the high-risk departments in surgical medicine were neurosurgery depart-ment,otolaryngology department and orthopedics department;the high-risk departments in gynecology and pediatrics system were obstetrics and gynecology.Conclusion The risk assessment model of nosocomial infection based on risk matrix truly and objec-tively reflects the risk of nosocomial infection in various departments of primary hospitals,which suggests that primary hospitals should give priority to the prevention and control strategy of nosocomial infection in high-risk general departments,and pay atten-tion to key departments such as neonatology.