中药多成分体系谱动学数学模型的实验验证研究
Validation of chromatogram pharmacokinetic model, a pharmacokinetics for multiple components in Chinese materia medica with fingerprint chromatography
邓俊林 1邓凯文 2刘文龙 3石继连 3杨岩涛 3罗杰英 4贺福元5
作者信息
- 1. 湖南中医药大学,长沙410208;湖南中医药大学现代中药制技术与评价实验室,长沙410208
- 2. 湖南中医药大学第一附属医院,长沙410007
- 3. 湖南中医药大学,长沙410208;中药药性与药效国家中医药管理局重点实验室,长沙410208;湖南中医药大学中医药超分子机理与数理特征化实验室,长沙410208
- 4. 湖南中医药大学,长沙410208
- 5. 湖南中医药大学,长沙410208;中药药性与药效国家中医药管理局重点实验室,长沙410208;湖南中医药大学现代中药制技术与评价实验室,长沙410208;湖南中医药大学中医药超分子机理与数理特征化实验室,长沙410208
- 折叠
摘要
目的:对已建立以指纹图谱表达的中药多成分体系的药物动力学,亦谱动学的总量统计矩数学模型进行实验验证.方法:以补阳还五汤中黄芪甲苷、芍药苷与苦杏仁苷3成分为模型药物,采用反相高效液相色谱法测定,分别用成分叠加拟合、总量统计矩、线性谱动学、梯形谱动学四计算法求算出3成分的谱动学总量统计矩参数.结果:其中线性谱动学计算的总量统计矩参数分别为VUCr为(118.8-±14.56) mg·min· mL-1;MRTT为(168.6±6.564) min; VRTr为(89 670±2 089) min2; ClT为(0.4556±0.03505)mL·min-1·kg-1; VT为(76.90±5.238) mL/kg;t0.95T为(622.2±16.89) min; λ0.95T为(106.1±0.6339)min.结论:3成分总量的半衰期为(116.8±5.503) min,3药在0-622.2min内代谢95％的浓度.四法参数无显著性差异.所建立的中药谱动学数学模型及参数体能表征中药多成分体系药物动力学行为.
Abstract
Objective:To experimentally study on the pharmacokinetics for the multiple components in the Chinese materia medica (CMM) with fingerprint chromatography,as total quantum statistic moment model (TQSMM) in Chromatogram pharmacokinetics.Methods:The RP-HPLC was used with a mobile phase of acetonitrile-water.The parameters of pharmacokinetics for single components were dealt with DAS and the TQSM parameters calculated by Excel software written as equations.The parameter ingredient fitting,total quantum moment,linear Chromatogram pharmacokinetics,trapezoid chromatogram pharmacokinetics.Results:The total quantum parameters had shown VUCr (118.8±14.56)mg·min·mL-1; MRTr (168.6±6.564)min; VRTr (89 670±2 089)min2; Clr (0.4556±0.03505) mL.min-1·kg-1; Vr (76.90±5.238)mL/kg; t0.95r (622.2±16.89)min; λ0.95r (106.1±0.6339)min.Conclusion:It is suggested that the half time for three ingredients was (116.8±5.503) min with 95％of ingredient metabolic period as 0-622.2min; Analyses are carried out with no significant difference by four calculation ways such as overlapped among the linear chromatogram pharmacokinetics,the chromatogram pharmacokinetic model and parameter system can be utilized to study on multiple component pharmacokinetic behavior.
关键词
中药/谱动学/药物动力学/总量统计矩/补阳还五汤/指纹图谱/数学模型Key words
Chinese materia medica/Chromatogram pharmacokinetics/Pharmacokinetics/Total quantum statistic moment/Buyang Huanwu Decoction/Fingerprint chromatographic/Mathematical model引用本文复制引用
基金项目
国家自然科学基金(81073142)
国家自然科学基金(81173558)
教育部高等学校博士学科点专项科研基金(20124323110002)
湖南省自然科学基金重点项目(11JJ2055)
湖南省教育厅十二五药学重点学科()
出版年
2013
国家科技期刊平台
NSTL
维普
万方数据