Rearch on aqueous extract of Taxus chinensis var.mairei (AETC) induces apoptosis of human lung carcinoma A549 cells through EGFR/MAPK pathway
Objective:The study aimed at further investigating the inhibition efficacy of aqueous extract of Taxus chinensis var.mairei (AETC) on human lung cancer A549 cells proliferation and induce its apoptosis and the possible mechanisms.Methods:MTT method was used to detect the inhibition of AETC on A549 cell proliferation inhibition.Flow cytometry assays were performed to evaluate the effects of AETC on the apoptosis of A549 cells.Western Blot analysis was applied to detect the related protein expression of EGFR/MAPK signal pathway.Results:AETC inhibited the proliferation of A549 cells in a concentration-dependent manner.The median inhibition concentration (IC50) values were 2.20mg/mL.The apoptotic rates of 2.0,2.5,3.0mg/mL AETC treatments for 24h were 20.9％,38.0％ and 61.7％,respectively.AETC treatment strongly reduced the level of phospho-EGFR and phospho-ERK1/2 while increased phospho-p38.However,no significant effects on the expression of EGFR,ERK1/2 and p38 MAPK were detected.Conclusion:AETC inhibites the proliferation of A549 cells through multiple targets.Inhibiting EGFR/MAPK signaling pathways induce its apoptosis is one of its possible mechanism.
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