Study on the fingerprint and chemical pattern recognition method for Pan'an atractylodes phizome based on UPLC
Objective:Establishing the fingerprint of Pan'an atractylodes phizome with UPLC method to evaluate the quality through the similarity calculation and chemical pattern recognition.Methods:Separation was performed at 25℃ on an Acquity UPLC BEH C18 column (2.1mm×50mm,1.7μm).Gradient elution was performed with the mobile phase of methanol and water.The flowing rate was 0.25mL/min.The UV detective wavelength was set at 240rim.The sample size was 10μL.Total 19 batches of Pan'an atractylodes rhizome and 14 batches of atractylodes rhizome from any other areas were analyzed.The similarity was calculated,then the cluster analysis and principal component analysis were analyzed.Results:The UPLC fingerprint was developed and the reference chromatographic fingerprint of Pan'an atractylodes rhizome was established.The results of similarity calculation were more than 0.95,which were in accordance with the results of the cluster analysis and principal component analysis.Conclusion:The fingerprint of atractylodes phizome established has a high characteristic,which could be used in the quality evaluation of atractylodes phizome if combined with pattern recognition that would provide a reference for the quality control of atractylodes phizome.
NETL
NSTL
万方数据
维普
