Influence of Wuhu Decoction on airway remodeling of young rat with asthma induced by virus
To investigate the effect of Wuhu Decoction on airway remodeling of young SD rat model with asthma induced by virus.Methods:48 rats were randomly divided into 6 groups:control group (Group K),asthma model group (Group M),high,middle,low dose of Wuhu Decoction groups (Group G,Z,D),positive control group (Group Y).The rat model of asthma was established by using sensitization of RSV combined with OVA.Rats in Group G,Z and D received gavage administration with high,middle and low dose of Wuhu Decoction,respectively,rats in Group K and M received gavage administration with normal saline,and rats in Group Y received gavage administration with dexamethasone.After treating for two weeks,the right lung tissues of rats were obtained and used to observe the pathologic changes of airways,deposition and expression of collagen fibers,expression of IL-13 by using HE staining,Masson staining and Western blot,respectively.Correlation between the two factors was analyzed.Results:Compared with Group K,the inflammatory cell infiltration of rats in Group M was increased,and the changes of airway remodeling appeared in rats of Group M such as subepithelial collagen deposition,smooth muscle cell layer thickening and so on.Compared with Group M,the degree of airway remodeling of rats in Group G,Z,and Y were significantly alleviated,while the changes of rats in Group D were not significant.Compared with Group K,the IOD value of collagen deposition and IL-13 level of rats in Group M were significantly increased (P＜0.01).The above changes of rats in Group G,Z and Y were significantly lower than those of Group M (P＜0.01),and the difference between Group M and Group D was not significant.The IOD value of collagen deposition was positively related to the expression of IL-13 in lung tissue.Conclusion:The high and middle dose of Wuhu Decoction could reduce the collagen deposition in lung tissue by down-regulating the expression of IL-13 to inhibit the airway remodeling in asthma rats.
NETL
NSTL
万方数据
维普
