首页|消癌解毒方含药血清对人肝癌SMMC-7721细胞端粒酶及凋亡相关基因mRNA表达的影响

消癌解毒方含药血清对人肝癌SMMC-7721细胞端粒酶及凋亡相关基因mRNA表达的影响

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目的:探讨消癌解毒方含药血清对人肝癌细胞SMMC-7721细胞端粒酶及凋亡相关基因mRNA表达的影响.方法:消癌解毒方含药血清作用于人肝癌SMMC-7721细胞,ELISA试剂盒检测各组端粒酶浓度,采用实时荧光定量PCR方法检测药物作用前后各组SMMC-7721细胞Bax、Bcl-2、CytC、Caspase-3 mRNA表达水平.结果:消癌解毒方含药血清作用于人肝癌SMMC-7721细胞可以降低其端粒酶浓度,且呈剂量依赖性;可使Bcl-2 mRNA表达水平下调,使Bax、CytC、Caspase-3 mRNA表达水平上调,下调Bcl-2/Bax mRNA比例,且中、高剂量组与对照组比较效果明显(P＜0.01,P＜0.05).结论:消癌解毒方含药血清可能通过抑制端粒酶活性,调控凋亡相关基因mRNA的表达,抑制人肝癌SMMC-7721细胞生长,促使其凋亡,在肝癌治疗中发挥疗效.
Effects of Xiao'ai Jiedu Formula drug-containing serum on telomerase and apoptosis-related mRNA expression in human hepatoma SMMC-7721 cells
Objective:To study the effects of Xiao'ai Jiedu Formula drug-containing serum on telomerase and apoptosis-related mRNA expression in human hepatoma SMMC-7721 cells.Methods:The effects of Xiao'ai Jiedu Formula drug-containing serum on human hepatoma SMMC-7721 cells was observed.The concentration of telomerase in each groups were measured by ELISA kits,and the expression of Bax,Bcl-2,CytC and Caspase-3 mRNA in SMMC-7721 cells were measured by Real Time PCR method before and after medication.Results:Xiao'ai Jiedu Formula drug-containing serum could reduce the telomerase concentration of human hepatoma SMMC-7721 cells and the efficacy was dose-dependent.It was also showed that Xiao'ai Jiedu Formula can down-regulate the Bcl-2 mRNA expression,up-regulate the expression of Bax,Cyt C,Caspase-3 mRNA,and decrease the Bcl-2/Bax mRNA ratio.The efficacy of Xiao'ai Jiedu Formula was more obvious in the middle and high-dose groups than in the control group (P＜0.01,P＜0.05).Conclusion:Xiao'ai Jiedu Formula is able to inhibit the growth of human hepatoma SMMC-7721 cells and promote the apoptosis by suppressing telomerase activity and regulating the expression of apoptosis-related mRNA,thus playing a part in treating liver cancer.

Xiao'ai Jiedu FormulaHuman hepatoma SMMC-7721 cellsTelomeraseReal time PCRApoptosisBaxBcl-2CytCCaspase-3

李文婷、赵凤鸣、周红光、周韬、李沐涵、李黎、吴勉华

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南京中医药大学,江苏省中医药防治肿瘤协同创新中心,南京210023

消癌解毒方 人肝癌SMMC-7721细胞 端粒酶 实时荧光定量PCR 凋亡 Bax Bcl-2 CytC Caspase-3

国家自然科学基金面上项目国家自然科学基金面上项目国家自然科学基金面上项目国家自然科学基金青年项目中华人民共和国教育部课题江苏省普通高校研究生科研创新计划省立项目

8127371781473608813735118110256320113237110001CXLX12_0572

2016

中华中医药杂志
中华中医药学会

中华中医药杂志

CSTPCDCSCD北大核心
影响因子：1.135
ISSN：1673-1727
年,卷(期)：2016.31(4)
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