GC-MS analysis and antimicrobial activity study of volatile oil extracted from Alpinia sichuanensis leaves
Objective:To analyze the chemical components of volatile oil extracted from Alpinia sichuanensis leaves and assay the antimicrobial activities.Methods:The volatile oil was extracted by the steam distillation method.The chemical components of volatile oil were analyzed by GC-MS.The minimal inhibitory concentrations of volatile oil on Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa and Candida albicans were determined by microplate assay.The minimum inhibitory concentration of volatile oil on Aspergillus niger was determined by drug plate assay.Results:A total of 54 peaks in GC-MS were separated,where 39 compounds were identified.And,the main component was the eucalyptol accounting for 41.86％.The minimal inhibitory concentration of volatile oil extracted from Alpinia sichuanensis leaves on Staphylococcus aureus was 250μg/mL.Conclusion:The volatile oil extracted form Alpinia sichuanensis leaves had an inhibitory effect on the Staphylococcus aureus.This current study will help to provide a scientific basis for the further research and utilization of Alpinia sichuanensis.
NETL
NSTL
万方数据
维普
