Trichosanthes pericarpium enhances the mobilization of CD34+ cells via matrix metalloproteinase 9 in the rat model of hyperlipemia complicated with acute myocardial ischemic
Objective:To investigate the effect of the Chinese herbal medicine trichosanthes pericarpium on the mobilization of CD34+ cells in hyperlipemia complicated with acute myocardial ischemia (HL-AMI) rats,and to reveal the mechanism in the perspective of regulating marrow stem cells mobilization.Methods:Wistar male rats were randomly divided into the control group,model group and the trich group,moreover,rats in each group were randomly redivided into 7 subgroups.Rats in the 1st-7th subgroup were phlebotomized on the lst-7th day after AMI model established respectively.CD34+ cells and the level of matrix metalloproteinase 9 (MMP-9) in the serum and in the myocardial tissue of each rat were determined.Results:During 7 days after AMI operation,in rats of the model group,the number of CD34+ cells increased rapidly during the 1st-6th day,reached its peak value and decreased obviously on the 6th day (P＜0.05);the serum level of MMP-9 increased rapidly since the 3rd day,and decreased obviously since the 4th day (P＜0.01).However,within the same phase,in the rats of the trich group,the blood contents of CD34+ cells and MMP-9 always increased,and were higher than those levels in the rats of model group during the whole experiment phase (P＜0.05).Conclusion:In AMI rats,the trichosanthes pericarpium water decoction could significantly promote the mobilization of CD34+ cells and notably upregulate the expression of MMP-9,and the mechanism may be related to upregulates MMP-9 activity and protein expression
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