葛根芩连汤对2型糖尿病大鼠脂肪组织Socs3甲基化影响及其与生理生化指标的相关性
Effects of Gegen Qinlian Decoction on methylation of Socs3 gene in adipose tissue from T2DM rats and correlations between methylation and physiological and biochemical parameters
彭淑红 1刘欣宜 2黄鑫 2李紫薇 2曾伟红 2徐国良 1朱卫丰 2姚鹏程2
作者信息
- 1. 江西中医药大学,南昌 330004;江西省中医病因生物学重点实验室,南昌 330004
- 2. 江西中医药大学,南昌 330004
- 折叠
摘要
目的:探讨葛根芩连汤(GQD)对链脲佐菌素和高脂饲料诱导的2型糖尿病(T2DM)大鼠脂肪组织Socs3甲基化的作用及其与生理生化指标的相关性.方法:40只SD大鼠随机分为空白组,模型组,GQD低、高剂量(5.5、11.0 g/kg)组(GQDL、GQDH组),各10只.取附睾脂肪组织,测定Socs3表达和基因启动子附近片段C甲基化水平;并分析C甲基化与基因表达量及大鼠生理生化指标之间的相关性.结果:GQDL组和GQDH组均能显著逆转模型组大鼠Socs3表达的降低(P＜0.01);与空白组比较,模型组Socs3基因CG3(GATA1结合位点)甲基化水平显著增加(P＜0.05),且CG3甲基化水平与GSP呈显著正相关(ρ=0.413,P=0.045),与TC、HDL-c呈显著负相关(ρ=-0.432,P=0.035),但GQDH和GQDL均不能有效逆转该位点甲基化.结论:GQD能有效逆转T2DM大鼠附睾脂肪组织Socs3表达;Socs3基因启动子附近CG3位点甲基化与GSP、TC和HDL-c有相关性,但该位点可能不是GQD的作用靶点.
Abstract
Objective:To explore the effects of Gegen Qinlian Decoction(GQD)on methylation of Socs3 gene in the adipose tissue of rats with type 2 diabetes mellitus(T2DM)induced by streptozotocin and high-fat diet as well as the correlations between methylation and physiological and biochemical indicators.Methods:Forty rats were divided into four groups,namely,the control group,the model group,low-(5.5 g/kg),and high-dose(11.0 g/kg)GQD(GQDL and GQDH)groups,10 rats in each group.The epididymal adipose tissue of rats was collected for detecting mRNA level and all the cytosine methylation levels.Spearman correlation coefficient was used to analyze the correlations between C methylation in the amplified fragment and physiological and biochemical indicators.Results:GQDL and GQDH remarkably reversed the down-regulated mRNA level of Socs3 triggered by STZ and HFD(P＜0.01).The higher percent methylation at CG3[part of the binding site of GATA binding protein 1(GATA1)]was found in model group compared to control group(P＜0.05).Furthermore,methylation level of CG3 had significant positive correlations with glycosylated serum protein(GSP)(ρ=0.413,P=0.045)and inverse correlation with total cholesterol(TC)and high density lipoprotein cholesterol(HDL-c)(ρ=-0.432,P=0.035).However,the methylation of CG3 showed no significant difference between Model and GQDL or GQDH.Conclusion:GQD reverses effectively the expression of Socs3 in epididymal adipose tissue of T2DM rats.CG3 methylation near the promoter in Socs3 is associated with GSP,TC,and HDL-c,but this site may not be a target for GQD.
关键词
葛根芩连汤/2型糖尿病/Socs3/DNA甲基化Key words
Gegen Qinlian Decoction/Type 2 diabetes mellitus/Socs3/DNA methylation引用本文复制引用
基金项目
国家自然科学基金(81503315)
国家自然科学基金(82060741)
出版年
2024
NETL
NSTL
万方数据