Dicussion on the effects of electroacupuncture on the learning and memory abilities of Alzheimer's disease rats based on the TLR4/MyD88/NF-κB signaling pathway
Objective:To observe the effects of electroacupuncture(EA)at'Shenshu'(BL 23)and'Fenglong'(ST 40)on learning and memory ability in Alzheimer's disease(AD)rats,and to explore its mechanism based on Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear factor κB(NF-κB)signaling pathway.Methods:A total of 60 male SD rats were randomly divided into sham operation,model,tonifying kidney,resolving phlegm and tonifying kidney and resolving phlegm groups.Except for the sham-operation group,the rats in the other groups were injected with β-amyloid(Aβ)25-35 into bilateral ventricles to establish the model.EA was applied to'Shenshu'(BL 23),'Fenglong'(ST 40),'Shenshu'(BL 23)and'Fenglong'(ST 40)in the Bushen,Huatan and Bushen Huatan group,respectively.Morris water maze(MWM)was used to detect learning and memory ability.HE staining and Nissl staining were used to observe the pathological morphology of hippocampus.ELISA was used to detect the concentrations of tumor necrosis factor(TNF)-α,interleukin(IL)-1 β,IL-4,IL-6 and IL-10 in serum.The expressions of TLR4,MyD88,NF-KB p65,TNF-α,IL-1β and IL-6 mRNA in hippocampus were detected by RT-PCR.The protein expressions of TLR4,MyD88 and NF-κB p65 in hippocampus were detected by Western Blot.Results:Compared with the model group,the average escape latency of the other groups was significantly reduced(P＜0.01),and the average number of platform crossings significantly increased(P＜0.01);The hippocampal neuronal hierarchy was clear,and the relative expression of neurons was significantly increased(P＜0.01);The serum levels of TNF-α,IL-1β and IL-6 were significantly decreased(P＜0.01),and the concentrations of IL-4 and IL-10 were significantly increased(P＜0.01);The relative expressions of TNF-α,IL-1β,IL-6,TLR4,MyD88 and NF-κB p65 mRNA in hippocampus were significantly decreased(P＜0.05);The relative expressions of TLR4,MyD88,and NF-κB p65 proteins in the hippocampus were significantly decreased(P＜0.01).Conclusion:EA can improve the learning and memory ability of AD rats,which may be related to down-regulating TLR4/MyD88/NF-κB signaling pathway and inhibiting inflammatory response.
Alzheimer's diseaseElectrioacupunctureLearning and memoryTLR4/MyD88/NF-κB signaling pathway
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