大鼠膀胱经循经推拿按压仪的研制及对焦虑大鼠模型效应的研究
Study on development of a rat bladder meridian massage and pressing instrument and its effect on an anxiety rat model
陆萍 1郭光昕 2张帅攀 1杨玉真 1何佩 1李晓峰 3房敏4
作者信息
- 1. 上海中医药大学针灸推拿学院,上海 201203
- 2. 上海中医药大学针灸推拿学院,上海 201203;上海中医药大学附属市中医医院,上海 200071
- 3. 济南益延科技有限公司,济南 250101
- 4. 上海中医药大学针灸推拿学院,上海 201203;上海中医药大学附属岳阳中西医结合医院,上海 200437;上海市中医药研究院推拿研究所,上海 200437
- 折叠
摘要
目的:研制智能化模拟手法参数仪器,探索仪器对焦虑大鼠的抗焦虑作用机制.方法:采用YLS-34A大鼠膀胱经循径推拿按压仪(简称YLS-34A按压仪),针对30只SD大鼠,即对照组、模型组、膀胱经推拿组(BMM组),进行干预.干预后比较大鼠的糖水偏好试验、尾悬架试验、强迫游泳试验行为学;检测海马炎症小体的NLRP3/NF-κB信号蛋白的变化.结果:YLS-34A按压仪能有效固定大鼠,自动化准确标定大鼠背部穴位,改善大鼠糖水偏好、悬尾不动时间、强迫游泳时间等焦虑样行为;显著降低焦虑症大鼠海马NLRP3/NF-kB信号通路蛋白表达(P＜0.05).结论:YLS-34A大鼠按压仪能为大鼠实验量化操作提供一种可靠工具,膀胱经按压对大鼠焦虑样行为的改善作用与抑制海马NLRP3/NF-κB信号通路有关.
Abstract
Objective:An intelligent simulation technique parameter instrument was developed to explore the anxiolytic action mechanism of the instrument on anxious rats.Methods:Using YLS-34A Rat Bladder Meridian Massage and Pressing Instrument(referred to as YLS-34A Massage and Pressing Instrument)to simulate the rats.Thirty SD rats were randomly divided into a control group,a model group,and a bladder massage group(Massage of bladder meridian,BMM group).After intervention,compared the behavior of sugar water preference test,tail suspension test and forced swimming test;detect the related protein pathway of hippocampal inflammasome.Results:YLS-34A Massage and Pressing Instrument can effectively fix rats,automatically and accurately map back acupoints and improve anxiety-like behavior;significantly reduce the anxiety rats'anxiety-like behaviors such as sugar water preference,hanging tail motionless time,forced swimming time,etc.;significantly reduce the protein expression of NLRP3/NF-κB signaling pathway in hippocampus of anxiety rats(P＜0.05).Conclusion:YLS-34A Massage and Pressing Instrument can provide a reliable tool for the improvement of anxiety-like behavior.It is related to the inhibition of hippocampal NLRP3/NF-κB signaling.
关键词
焦虑症/膀胱经推拿/动物模型/按压手法仪/炎症小体Key words
Anxiety disorder/Bladder meridian massage/Animal model/Pressing manipulative instrument/Inflammasome引用本文复制引用
基金项目
国家自然科学基金面上项目(81973974)
国家自然科学基金重点项目(82030121)
上海市"超级博士后"项目(2020371)
出版年
2024
NETL
NSTL
万方数据