Effect and correlation analysis of Yangxin Tongmai Prescription on intestinal mucosal flora,serum inflammatory factors and endotoxin in coronary heart disease rats with blood stasis syndrome
Objective:To investigate the effect and correlation analysis of Yangxin Tongmai Prescription on changes of intestinal mucosal flora,serum inflammatory factors and endotoxin in coronary heart disease with blood stasis syndrome rats.Methods:A total of 40 male SD rats were randomly divided into normal group(n=8)and model building group(n=32).The model group were fed with high-fat diet for 3 months,combined with vitamin D3 intragastric administration and isoproterenol subcutaneous injection to construct coronary heart disease with blood stasis syndrome rats.Then the model building group were randomly divided into 3 groups:model group,Yangxin Tongmai Prescription group(12 g·kg-1·d-1),atorvastatin group(1.8 mg·kg-1·d-1),were given intragastric administration for 2 weeks.After the intervention,the small intestinal mucosal tissues were sequenced by 16S rRNA and detected by transmission electron microscopy;HE staining was used to observe the myocardium;Serum Tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-10(IL-10)and endotoxin were detected by ELISA.Results:Compared with the normal group,the electrocardiograph T-wave of the model building group was higher and the ST segment was significantly elevated(P＜0.01),heart rate decreased(P＜0.01).HE staining myocardial fiber necrosis dissolution,a large number of inflammatory cells infiltrated.The lesions in the Yangxin Tongmai Prescription group and atorvastatin group were less than those in the model group.Compared with the normal group,the contents of TNF-α,IL-6,endotoxinin serum of model group were increased(P＜0.01),but IL-10 was decreased(P＜0.01).Compared with the model group,the contents of TNF-α and endotoxin in Yangxin Tongmai Prescription group were decreased(P＜0.01),while IL-10 was increased(P＜0.01).The relative abundance of Ralstonia increased in the model group(P＜0.05),but decreased in Yangxin Tongmai Prescription group,although the difference was not statistically significant(P=0.06).The abundance of Unspecified_Peptostreptococcaceae in model group decreased(P＜0.0l).Pearson correlation analysis showed that the abundance of Ralstonia was positively correlated with TCHO,HDL-C,LDL-C,hemorheology,TNF-α(P＜0.05),and negatively correlated with IL-10(P＜0.05).The abundance of Unspecifed_Peptostreptococcaceae and Unspecifed_Clostridiales were negatively correlated with TCHO,HDL-C,LDL-C,hemorheological indexes,TNF-α and endotoxin(P＜0.05,P＜0.01),positively correlation with IL-10(P＜0.05).Transmission electron microscopy of ileum tissue showed that mitochondria lesion,cell nucleus fragmentation and microvilli sparsity were observed in model group.No obvious lesions were observed in Yangxin Tongmai Prescription group and atorvastatin group.Conclusion:The microflora of small intestinal mucosa was correlated with TNF-α,IL-10,endotoxin,blood lipid and hemorheology in rats with blood stasis syndrome of coronary heart disease.Yangxin Tongmai Prescription can improve the blood stasis syndrome of coronary heart disease by possibly regulating the intestinal mucosal microflora and reducing the levels of TNF-α and endotoxin in serum.
Intestinal floraCoronary heart diseaseBlood stasis syndromeTraditional Chinese medicineEndotoxin
NETL
NSTL
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