Study on the mechanism of Shaoyao Gancao Decoction in repairing the damage of PM2.5-induced systemic lupus erythematosus mouse
Objective:Established a systemic lupus erythematosus(SLE)mouse model exposured by PM2.5,and to study the therapeutic effect and mechanism of Shaoyao Gancao Decoction(SGT)on PM2.5 toxicity and pathological injury of(SLE)mice.Methods:A total of 40 C57BL/6 female mice were randomly divided into blank control group,SLE group,SLE+PM2.5 group(model group)and SGT group.Model group was exposed to PM2.5 to establish a mouse model of SLE.After treatment,the serum levels of anti-ds-DNA were measured by ELISA;the morphological changes of kidney and lung were observed by HE staining;the content of reactive oxygen species(ROS)in bronchoalveolar lavage fluid(BALF)was detected by ELISA.The related protein NF-κB p65,p-NF-κB p65,TLR4,COX-2 expression levels were detected by Western Blot.Collected mouse feces and analyzed the diversity and difference of intestinal microflora by 16S rRNA.Results:Compared with the model group,after treated with SGT,spleen index and the serum level of anti-ds-DNA were significantly decreased(P＜0.01);the glomerular basement membrane was uniform and the foot process was slightly fused in SGT group;the ROS content in BALF decreased significantly(P＜0.05);HE staining section showed that the inflammatory cell exudation was reduced,the inflammation related protein expression of NF-κB p65,p-NF-κB p65,TLR4,COX-2 were significantly decreased(P＜0.05).The results of 16S rRNA gene analysis showed that the intestinal microflora of mice in each group was diverse and the structure of each group was different(P＜0.05).The diversity of intestinal microflora was increased after treatment of SGT,and the abundance of protective bacteriawas up-regulated(P＜0.05).Conclusion:PM2.5 can exacerbate the incidence of SLE,SGT can improve the pathological damage of mice with SLE after PM2.5 exposure,the mechanism of action may be that it plays a therapeutic role in SLE by repairing PM2.5-induced inflammation and intestinal flora disorder.
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