熟地黄抗光诱导的光感受器退行性病变效应研究
Study on the effects of Rehmanniae Radix Praeparata on photoreceptor degeneration induced by light
李浩闻 1徐静 2杜霄烨 2崔金刚 2马荣 2张腾 2陈瑜2
作者信息
- 1. 上海中医药大学附属岳阳中西医结合医院,上海 200437
- 2. 上海中医药大学附属岳阳中西医结合医院,上海 200437;上海市中医药研究院中西医结合临床研究所,上海 200437
- 折叠
摘要
目的:采用光损伤小鼠模型,探究熟地黄对光感受器的保护效应.方法:25只雌性Balb/c小鼠,随机分为正常对照组,光损伤模型组,熟地黄低、中、高剂量组,各5只.光损伤模型组及熟地黄各剂量组接受(15 000±10)lux白光光照30 min进行造模.通过结构、形态、功能以及分子病理等方面,评价熟地黄的光感受器保护作用.结果:OCT和HE染色结果表明,熟地黄呈剂量依赖性显著保护光损伤小鼠视网膜结构(P＜0.05);ERG分析结果表明,熟地黄可显著保护光损伤小鼠视网膜功能(P＜0.05);免疫组织化学分析结果表明,熟地黄可显著保护光感受器一级神经元及二级神经元结构并抑制Müller细胞反应性增生(P＜0.05).结论:熟地黄可显著抑制光损伤诱导的光感受器退行性改变及相关视网膜二级神经元损伤,同时对Müller细胞反应性异常增生具有显著的抑制作用.
Abstract
Objective:To investigate the photoreceptor protective effects of Rehmanniae Radix Praeparata(RRP)in a light damaged mouse model.Methods:A total of 25 female Balb/c mice were randomly divided into normal control group,light damage model group,low-,medium-and high dose-RRP group.The light damage model group and each dose group of RRP were exposed to(15 000±10)lux of white light for 30 minutes for modeling.To directly evaluate the photoreceptor protective effect of RRP from aspects such as structure,morphology,function,and molecular pathology.Results:OCT and HE staining results showed that RRP significantly protected the retinal structure of mice with light damage in a dose dependent manner(P＜0.05).The results of ERG analysis showed that RRP can significantly protect the retinal function of mice with light damage(P＜0.05).Immunohistochemical analysis showed that RRP can significantly protect the structure of photoreceptor neurons and secondary neurons,and inhibit the reactive proliferation of Müller cells(P＜0.05).Conclusion:The intervention of RRP can significantly inhibit photoreceptor degeneration induced by light damage and related retinal secondary neuron damage,and has a significant inhibitory effect on the reactive proliferation of Müller cells.
关键词
熟地黄/光感受器/视网膜/光感受器退行性病变Key words
Rehmanniae Radix Praeparata/Photoreceptors/Retina/Photoreceptor degeneration引用本文复制引用
基金项目
国家自然科学基金面上项目(81673790)
上海市科学技术委员会优秀学术带头人计划(19XD1403700)
出版年
2024
NETL
NSTL
万方数据