Study on the effects of Rehmanniae Radix Praeparata on photoreceptor degeneration induced by light
Objective:To investigate the photoreceptor protective effects of Rehmanniae Radix Praeparata(RRP)in a light damaged mouse model.Methods:A total of 25 female Balb/c mice were randomly divided into normal control group,light damage model group,low-,medium-and high dose-RRP group.The light damage model group and each dose group of RRP were exposed to(15 000±10)lux of white light for 30 minutes for modeling.To directly evaluate the photoreceptor protective effect of RRP from aspects such as structure,morphology,function,and molecular pathology.Results:OCT and HE staining results showed that RRP significantly protected the retinal structure of mice with light damage in a dose dependent manner(P＜0.05).The results of ERG analysis showed that RRP can significantly protect the retinal function of mice with light damage(P＜0.05).Immunohistochemical analysis showed that RRP can significantly protect the structure of photoreceptor neurons and secondary neurons,and inhibit the reactive proliferation of Müller cells(P＜0.05).Conclusion:The intervention of RRP can significantly inhibit photoreceptor degeneration induced by light damage and related retinal secondary neuron damage,and has a significant inhibitory effect on the reactive proliferation of Müller cells.
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