Effects of drug properties and irritation in fermentation and processing of Arisaema Cum Bile
Objective:By comparing the drug properties and irritating changes of Arisaematis Rhizoma and Arisaema Cum Bile,to provide modern pharmacological research basis for the traditional theory of'reducing toxicity and changing drug properties'of Arisaema Cum Bile fermentation and processing.Methods:A rat model of chronic obstructive pulmonary disease with the cold-phlegm in the lung syndrome(COPD-CPS)and acute exacerbation of chronic obstructive pulmonary disease with the heat-phlegm syndrome(AECOPD-HPS)was established,respetively,the drug groups were administered at a dose of 2.52 g/kg,continuously administered for 7 days,the body temperature and body mass changes of rats were observed.Epinephrine(E),the neutrophil elastin(NE),cortisol(Cor)and C-reactive protein(CRP)levels in rat serum and lung tissue mucin(MUC5AC),interleukin(IL)-8,tumor necrosis factor-α(TNF-α),eosinophils(EOS),neutrophils(NEU)in bronchoalveolar lavage were determinated by ELISA.Pathological state of lung tissue was determinated by HE staining.The effects of different drug concentrations on cell proliferation were detected by CCK-8,and the effects on cell irritation were investigated by ELISA method using the HaCaT cell supernatant inflammatory factors IL-1β,IL-8,transforming growth factor-β1(TGF-β1),serum soluble intercellular adhesion molecule-1(ICAM-1),and prostaglandin E2(PGE2).Results:In the model of COPD-CPS,compared with the model group,the Arisaematis Rhizoma group could significantly reduce NE,IL-8,TNF-α,MUC5AC and EOS(P＜0.01),the therapeutic effect of Arisaema Cum Bile group was not significant.In the model of AECOPD-HPS,compared with the model group,the Arisaema Cum Bile group significantly reduced the content of E,NE,Cor,CRP,IL-8,TNF-α,MUC5AC(P＜0.01),and increased the content of NEU(P＜0.01),the therapeutic effect of Arisaematis Rhizoma group was not significant.Compared with the blank control group,different concentration of the Arisaematis Rhizoma group significantly increased the contents of IL-1β,IL-8,TGF-β1,ICAM-1,and PGE2 in HaCaT cells(P＜0.01),while the above indicators were significantly reduced in all concentrations in the Arisaema Cum Bile group compared to the Arisaematis Rhizoma group(P＜0.01).Conclusion:After Arisaematis Rhizoma is processed into Arisaema Cum Bile by bile fermentation,its efficacy is transformed from warming and resolving cold phlegm to clearing away heat and resolving phlegm,and its toxicity is reduced,which verifies the traditional theory of'reducing toxicity and changing drug properties'of Arisaematis Rhizoma fermentation and processing into Arisaema Cum Bile.
Arisaematis RhizomaArisaema Cum BileCold-phlegm in the lung syndromeHeat-phlegm syndromeTraditional Chinese medicine processingFermentationIrritationDrug properties
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