红芪有效成分及其根际土壤无机元素累积特征研究
Study on the active constituents of Hedysari Radix and the accumulation characteristics of inorganic elements in rhizosphere soil
魏小成 1李成义 1周瑞娟 1张广袤 1贾妙婷 1罗旭东 1强正泽 1王明伟1
作者信息
- 1. 甘肃中医药大学药学院,兰州 730101
- 折叠
摘要
目的:探究不同生长期红芪有效成分及其根际土壤无机元素的累积变化特征,厘清生长期红芪无机元素的动态需求,为红芪适宜种植区域筛选及优化红芪种植技术提供依据.方法:不同生长期采集红芪及其根际土壤样品,通过火焰原子吸收光谱法(AAS)测定红芪根、茎、叶及根际土壤不同土层的无机元素含量,高效液相色谱法(HPLC)测定红芪根中异黄酮类成分含量,苯酚-硫酸法测定红芪总多糖的含量.采用相关性分析、主成分分析和正交偏最小二乘判别分析(OPLS-DA)等化学模式识别方法进行分析.结果:比较不同生长期红芪根、茎、叶及土壤中无机元素含量,发现红芪根、茎、叶中Cu元素含量稳定,20 cm土壤中Cu和K元素含量稳定;相关性分析表明,Ca元素-毛蕊异黄酮苷、Fe元素-红芪多糖之间存在显著负相关(P＜0.01);主成分分析及OPLS-DA结果表明Ca元素、Mg元素及Zn元素可能是不同生长期样品中主要变化的无机元素.结论:土壤无机元素可以影响生长期红芪有效成分积累,建议红芪选择Fe元素含量高和Zn元素含量低的土壤种植.
Abstract
Objective:To explore the accumulation characteristics of effective components of Hedysari Radix and inorganic elements in rhizosphere soil of Hedysari Radix in different growth stages,to clarify the dynamic demand of Hedysari Radix inorganic elements in the growth stage,and to provide a basis for screening suitable planting areas and optimizing planting techniques of Hedysari Radix.Methods:Hedysari Radix in different growth period and rhizosphere soil samples were collected.Hedysari Radix roots,stems,leaves and rhizosphere soil of different inorganic element content of soil were determinated by flame atomic absorption spectrometry(AAS),high performance liquid chromatography(HPLC)determinated isoflavone content in Hedysari Radix,phenol-sulfuric acid method was for determining the content of total polysaccharide Hedysari Radix.Chemical pattern recognition methods such as correlation analysis,principal component analysis and partial least squares discriminant analysis were used for analysis.Results:Inorganic elements contents in root,stem,leaf and soil of Hedysari Radix were compared in different growing stages.It was found that Cu content in root,stem and leaf of Hedysari Radix was stable,and Cu and K content in 20 cm soil was stable.Correlation analysis showed that there was a significant negative correlation between Ca element-calycosin glycosides and Fe-Hedysari Radix polysaccharide.The results of principal component analysis and partial least squares discriminant analysis showed that Ca,Mg and Zn were the main inorganic elements in different growth stages.Conclusion:Soil inorganic elements can affect the accumulation of effective components of Hedysari Radix during the growing period.It is suggested that Hedysari Radix should be planted in soils with low content of Fe and high content of Zn.
关键词
红芪/不同生长期/根际土壤/无机元素/累积特征/有效成分/种植技术Key words
Hedysari Radix/Different growth periods/Rhizosphere soil/Inorganic elements/Accumulation characteristics/Active constituents/Planting techniques引用本文复制引用
基金项目
国家自然科学基金地区项目(81860683)
国家自然科学基金地区项目(82160730)
国家自然科学基金青年科学基金项目(82104345)
甘肃省教育厅双一流重大科研项目(GSSYLXM-05)
&&(21YF5FA133)
出版年
2024
NETL
NSTL
万方数据