Effects of Baojin Chenfei Formula on fibrosis and lung tissue Akt/Gsk-3βpathway in silicosis rats
Objective:To explore the effects of Akt/Gsk-3 β pathway by Baojin Chenfei Formula(BJCF)in delaying fibrosis in silicotic rats.Methods:A total of 48 rats were randomly divided into normal group,model group,tetrandrine group and BJCF group,with 12 rats in each group.The silicosis rat model was established by one-time injection of SiO2 through trachea except for the normal group,the rats in each group were treated with the corresponding drugs on the day 15-28 after modeling,the rats were sacrificed and the samples were taken after treatment.After treatment,pulmonary function was measured,including VC,TV,PAU and PIF,the pathological morphology of lung tissue and score fibrosis were observed,Col-Ⅰ,Col-Ⅲ,hydroxyproline(HYP),N-cadherin,E-cadherin and Akt/Gsk-3 β pathway related mRNA and protein level in lung tissue were measured.Results:Compared with the normal group,the lung tissue structure was severely damaged and fibrous nodules were visible in model group,the PAU were significantly extended(P＜0.01),the fibrosis score,the content of HYP,the mRNA relative expression of α-SMA,TGF-β1,the protein relative expression of Col-Ⅰ,Col-Ⅲ,N-cadherin,p-Akt,p-Gsk-3β were significantly increased(P＜0.01,P＜0.05);the VC,TV and PIF and the mRNA,protein relative expression of E-cadherin were significantly decreased in the model group(P＜0.01,P＜0.05).Compared with the model group,the fibrosis score,the content of HYP,Col-I expression,and the protein relative expression of N-cadherin,p-Gsk-3 were significantly decreased in the BJCF group and the tetrandrine group(P＜0.01,P＜0.05),the protein relative expression of E-cadherin were significantly increased in the BJCF group and the tetrandrine group(P＜0.01),the Col-Ⅲ expression,PAU,α-SMA mRNA,TGF-β1 mRNA and the protein relative expression of p-Akt were significantly decreased in the BJCF group(P＜0.05,P＜0.01).Compared with tetrandrine group,the TV and the protein relative expression of E-cadherin were significantly increased in BJCF group(P＜0.05,P＜0.01),the Col-Ⅲ expression was significantly decreased in BJCF group(P＜0.05).Conclusion:BJCF can improve fibrosis in silicotic rats by inhibiting epithelial-mesenchymal transition,and its mechanism may be related to the regulation of Akt/Gsk-3β pathway.
SilicoticBaojin Chenfei FormulaSilicon dioxide(SiO2)Epithelial-mesenchymal transition(EMT)Akt/Gsk-3βpathwayMechanismPulmonary fibrosisPulmonary function
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