丹参酮ⅡA通过TGF-β1/PI3K/Akt信号通路对子宫内膜间质细胞纤维化的影响
Effects of tanshinone ⅡA on endometrial interstitial cells fibrosis through TGF-β1/PI3K/Akt signaling pathway
黄紫纯 1雷磊 1匡继林2
作者信息
- 1. 湖南中医药大学,长沙 410208
- 2. 湖南中医药大学第二附属医院,长沙 410005
- 折叠
摘要
目的:探讨丹参酮Ⅱ A(Tan Ⅱ A)通过转化生长因子-β1/磷脂酰肌醇-3-激酶/蛋白激酶B(TGF-β 1/PI3K/Akt)通路对人子宫内膜间质细胞(HESCs)纤维化的影响.方法:取10 ng/mL TGF-β 1诱导HESCs纤维化,将HESCs分为5组:正常对照组,模型组和TanⅡA低、中、高剂量组.CCK-8检测HESCs细胞毒性和增殖情况;免疫荧光检测α-平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原蛋白α1(Col1α1)荧光强度;Western Blot检测α-SMA、Col1 α1、.PI3K、Akt及p-Akt蛋白表达.结果:TanⅡA浓度在10～40 μ g/mL对HESCs无毒性.与正常对照组比较,模型组细胞增殖能力,α-SMA、Col1 α1荧光强度,α-SMA、Col1 α1、PI3K、p-Akt蛋白表达显著增多(P＜0.05,P＜0.01).与模型组比较,TanⅡA高剂量组细胞增殖能力显著下降(P＜0.05);各TanⅡA干预组α-SMA、Col1 α1 荧光强度,α-SMA、PI3K、p-Akt、Col1 α1 蛋白表达显著减少(P＜0.01,P＜0.05),各组 Akt 蛋白表达差异无统计学意义.结论:Tan ⅡA可通过抑制TGF-β1/PI3K/Akt信号通路有效抗子宫内膜纤维化.
Abstract
Objective:To investigate the effect of tanshinone ⅡA(TanⅡA)on human endometrial interstitial cells strain(HESCs)fibrosis through TGF-β1/PI3K/Akt signaling pathway.Methods:HESCs were infected with 10 ng/mL TGF-β1 to induce fibrosis,and HESCs was divided into 5 groups:normal control group,model group,Tan ⅡA low,medium,high dose group.The cytotoxicity and proliferation of HESCs were detected by CCK-8.The fluorescence intensity of α-SMA and Col1 α1 was detected by immunofluorescence.The protein expressions of α-SMA,Col1 α1,PI3K,Akt and p-Akt were detected by Western Blot.Results:The concentration of Tan ⅡA in 10-40 μg/mL had no toxicity to HESCs.Compared with normal control group,cell proliferation capacity,fluorescence intensity of α-SMA and Col1 α1,protein expression of α-SMA,Col1 α1,PI3K and p-Akt were increased in model group(P＜0.05,P＜0.01).Compared with model group,the cell proliferation ability of Tan ⅡA high dose group was decreased(P＜0.05).The fluorescence intensity of α-SMA and Col1 α1 and the protein expressions of α-SMA,PI3K,p-Akt and Col1 α1 decreased in each Tan ⅡA intervention group(P＜0.01,P＜0.05),but there were no significant differences in the expression of Akt among all groups.Conclusion:Tan ⅡA can effectively antiendometrial fibrosis by inhibiting the TGF-β1/PI3K/Akt signaling pathway.
关键词
宫腔粘连/丹参酮ⅡA/转化生长因子-β1/磷脂酰肌醇-3-激酶/蛋白激酶B信号通路/子宫内膜纤维化Key words
Intrauterine adhesions(IUAs)/Tanshinone ⅡA/Transforming growth factor-β1(TGF-β1)/PI3K/Akt signaling pathway/Endometrial fibrosis引用本文复制引用
基金项目
湖南省研究生科研创新项目(CX20200771)
湖南省自然科学基金项目(2021JJ30493)
湖南省卫生健康委项目(C202305016294)
出版年
2024
NETL
NSTL
万方数据