首页|丹参酮ⅡA通过TGF-β1/PI3K/Akt信号通路对子宫内膜间质细胞纤维化的影响

丹参酮ⅡA通过TGF-β1/PI3K/Akt信号通路对子宫内膜间质细胞纤维化的影响

Effects of tanshinone ⅡA on endometrial interstitial cells fibrosis through TGF-β1/PI3K/Akt signaling pathway

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目的:探讨丹参酮Ⅱ A(Tan Ⅱ A)通过转化生长因子-β1/磷脂酰肌醇-3-激酶/蛋白激酶B(TGF-β 1/PI3K/Akt)通路对人子宫内膜间质细胞(HESCs)纤维化的影响.方法:取10 ng/mL TGF-β 1诱导HESCs纤维化,将HESCs分为5组:正常对照组,模型组和TanⅡA低、中、高剂量组.CCK-8检测HESCs细胞毒性和增殖情况;免疫荧光检测α-平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原蛋白α1(Col1α1)荧光强度;Western Blot检测α-SMA、Col1 α1、.PI3K、Akt及p-Akt蛋白表达.结果:TanⅡA浓度在10～40 μ g/mL对HESCs无毒性.与正常对照组比较,模型组细胞增殖能力,α-SMA、Col1 α1荧光强度,α-SMA、Col1 α1、PI3K、p-Akt蛋白表达显著增多(P＜0.05,P＜0.01).与模型组比较,TanⅡA高剂量组细胞增殖能力显著下降(P＜0.05);各TanⅡA干预组α-SMA、Col1 α1 荧光强度,α-SMA、PI3K、p-Akt、Col1 α1 蛋白表达显著减少(P＜0.01,P＜0.05),各组 Akt 蛋白表达差异无统计学意义.结论:Tan ⅡA可通过抑制TGF-β1/PI3K/Akt信号通路有效抗子宫内膜纤维化.
Objective:To investigate the effect of tanshinone ⅡA(TanⅡA)on human endometrial interstitial cells strain(HESCs)fibrosis through TGF-β1/PI3K/Akt signaling pathway.Methods:HESCs were infected with 10 ng/mL TGF-β1 to induce fibrosis,and HESCs was divided into 5 groups:normal control group,model group,Tan ⅡA low,medium,high dose group.The cytotoxicity and proliferation of HESCs were detected by CCK-8.The fluorescence intensity of α-SMA and Col1 α1 was detected by immunofluorescence.The protein expressions of α-SMA,Col1 α1,PI3K,Akt and p-Akt were detected by Western Blot.Results:The concentration of Tan ⅡA in 10-40 μg/mL had no toxicity to HESCs.Compared with normal control group,cell proliferation capacity,fluorescence intensity of α-SMA and Col1 α1,protein expression of α-SMA,Col1 α1,PI3K and p-Akt were increased in model group(P＜0.05,P＜0.01).Compared with model group,the cell proliferation ability of Tan ⅡA high dose group was decreased(P＜0.05).The fluorescence intensity of α-SMA and Col1 α1 and the protein expressions of α-SMA,PI3K,p-Akt and Col1 α1 decreased in each Tan ⅡA intervention group(P＜0.01,P＜0.05),but there were no significant differences in the expression of Akt among all groups.Conclusion:Tan ⅡA can effectively antiendometrial fibrosis by inhibiting the TGF-β1/PI3K/Akt signaling pathway.

Intrauterine adhesions(IUAs)Tanshinone ⅡATransforming growth factor-β1(TGF-β1)PI3K/Akt signaling pathwayEndometrial fibrosis

黄紫纯、雷磊、匡继林

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湖南中医药大学,长沙 410208

湖南中医药大学第二附属医院,长沙 410005

宫腔粘连 丹参酮ⅡA 转化生长因子-β1 磷脂酰肌醇-3-激酶/蛋白激酶B信号通路 子宫内膜纤维化

湖南省研究生科研创新项目湖南省自然科学基金项目湖南省卫生健康委项目

CX202007712021JJ30493C202305016294

2024

中华中医药杂志
中华中医药学会

中华中医药杂志

CSTPCD北大核心
影响因子：1.135
ISSN：1673-1727
年,卷(期)：2024.39(2)
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