Effects of Wumei Pills on gene microenvironment expression profile and immune related gene expression in orthotopic tumor of breast cancer mice
Objective:To study the effect of Wumei Pills on the gene expression profile of in situ tumor microenvironment and immune related gene expression in breast cancer mice,and to explore the mechanism of Wumei Pills in inhibiting breast cancer.Methods:The mouse model of breast cancer was established by injecting 4T1 breast cancer cells into the second breast fat pad of mice.The model was divided into high-dose Wumei Pills group(32.88 g/kg),low-dose Wumei Pills group(16.44 g/kg),drug control group(Capecitabine,359 mg/kg)and blank control group.By observing the tumor volume,weight and inhibition rate of in situ breast cancer in mice,the tumor inhibition effect of the drug was judged.The characteristics of gene expression profiles in the microenvironment of orthotopic tumors were detected by gene expression profiling chip technology,and the GO and KEGG pathway characteristics of gene expression profiles were analyzed by bioinformatics methods.To compare the effect of Wumei Pills and Capecitabine on gene expression.RT-PCR,immunohistochemistry and Western Blot were used to analyze the expression of immune related genes affected by Wumei Pills.Results:Although the high-dose and low-dose Wumei Pills group were inferior to the drug observation group in tumor volume,weight and inhibition rate,there still showed a certain tumor inhibition effect compared with the blank control group.Among them,the effect of the low-dose Wumei Pills group was slightly better than that of the high-dose Wumei Pills group.After Wumei Pills intervention,there were 54 differential genes,including 30 up-regulated genes and 24 down-regulated genes.There were 104 differential genes after capecitabine intervention,including 54 up-regulated genes and 50 down regulated genes.There was only one difference gene between them.The genes regulated by Wumei Pills involved 152 GO items,while Capecitabine only involved 9 GO items.There were three KEGG pathways involved in Wumei Pills and one involved in Capecitabine.After Wumei Pills intervention,compared with the blank control group,the mRNA and protein expression of immune related genes Bmp4,Fzd9,Capn3,Fndc5 and Sfpq decreased,but the mRNA expression of Slc15a3,Tnfsf13b and Il1a increased,while the protein expression decreased.Conclusion:Wumei Pills can inhibit the growth of breast cancer to some extent.The effect of Wumei Pills on gene expression in the microenvironment of breast cancer tissue is completely different from that of Capecitabine,which involves more biological processes,cell components,molecular functions,and the regulation is more extensive.Wumei Pills can down regulate the expression of immune related genes Bmp4,Fzd9,Capn3,Fndc5 and Sfpq,and its effect on the expression of Slc15a3,Tnfsf13b and Il1a needs further analysis.It can be seen that the regulatory effect of Wumei Pills on gene expression in the microenvironment of breast cancer in situ may be one of the mechanisms of its anti-tumor effect.
Wumei PillsBreast cancerMicroenvironmentGene expression profileImmune function regulationMechanismBioinformatics
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