Effects of Huangqi Gegen Decoction combined with Gualou Decoction on SIRT1/AMPK signaling pathway in aorta of diabetic atherosclerosis rats
Objective:To explore the mechanism of Huangqi Gegen Decoction combined with Gualou Decoction in reducing blood glucose;blood lipid and oxidative stress in diabetes atherosclerotic rats based on the signal pathway of silent information regulator 1/AMP-activated protein kinase(SIRT1/AMPK).Methods:A total of 68 SPF male SD rats were randomly divided into blank group,model group,TMK group(Tangmaikang Granules),decoction group(Huangqi Gegen Decoction combined with Gualou Decoction),granule group(Huangqi Gegen Decoction and Gualou Decoction combined granule).Except for the blank group,each group was fed with fat diet for 28 days.On the 29th day,the rat model of diabetes atherosclerosis was induced by intraperitoneal injection of 2％STZ at the dose of 30 mg/kg.The corresponding drug or distilled water were given by gavage on the third day of injection of STZ,and the rats were gavaged continuously for 49 days.Biochemical analyzer detected blood glucose,triglyceride(TG),cholesterol(CHO),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),and ELISA detected the contents of propylene glycol(MDA),reactive oxygen species(ROS),superoxide dismutase(SOD)and glycosylated hemoglobin(HbA1c).Adiponectin receptor(AdipoR1),silent information regulator 1(SIRT1),adenylate activated protein kinase(AMPK),glucose transporter 4(GLUT4)and sterol regulatory element binding protein-1c(SREBP-1c)mRNA were detected by Real-time PCR.Results:Compared with the blank group,the contents of Glu,TG,CHO and LDL-C in the model group increased significantly(P＜0.05),the contents of HDL-C decreased significantly(P＜0.05),the contents of MDA,ROS and HbA1c increased(P＜0.05),the content of SOD decreased(P＜0.05),the expression of SIRT1,AMPK,GLUT4 and AdipoR1 mRNA decreased(P＜0.05),and the expression of SREBP-1c mRNA increased(P＜0.05).Compared with the model group,the giving drug groups could reduce the contents of Glu,TG,CHO,LDL-C,MDA,ROS and HbAlc in diabetes atherosclerotic rats(P＜0.05),increase the contents of HDL-C and SOD(P＜0.05),increase the mRNA of SIRT1,AMPK,GLUT4 and AdipoR1(P＜0.05),and reduce the expression of SREBP-1c mRNA(P＜0.05).Conclusion:Huangqi Gegen Decoction combined with Gualou Decoction reduce blood glucose and lipids in diabetes atherosclerotic rats,which may be related to stimulating the secretion of AdipoR1,activating SIRT1/AMPK signal pathway,and regulating the activities of downstream factors GLUT4 and SREBP-1c.Huangqi Gegen Decoction combined with Gualou Decoction may reduce the oxidative stress response by reducing ROS,MDA and increasing the expression of SOD and SIRT1.
DiabetesAtherosclerosisHuangqi Gegen DecoctionGualou DecoctionSIRT1AMPKMechanismOxidative stress response
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