Dynamic effect of Tuina rolling method on skeletal muscle injury repair in rabbits at various time points based on NF-κB signaling pathway
Objective:To investigate the mechanism of its effect on the repair of skeletal muscle injury by analyzing the effect of Tuina rolling method on NF-κB signaling pathway in rabbits with skeletal muscle injury at different time points.Methods:Seventy-two New Zealand rabbits were randomly divided into a blank group,a model group,and a rolling-method treatment group,with 24 rabbits in each group,and each group was further randomly divided into six subgroups(24 h,3 d,5 d,7 d,9 d,and 11 d after injury),with four rabbits in each subgroup.Gravity hammer blunt contusions were molded and treated with a homemade rolling-massage device for three consecutive days,2 times/day,3 min/time,at the six time points mentioned above.HE staining was collected 24 hours after the end of treatment to observe the histomorphological changes;ELISA was performed to detect the TNF-α and IL-1 β contents in the damaged tissues;Western Blot was performed to detect the NF-κB p65 protein and IκB α protein contents in the damaged tissues.Results:Inflammatory cell infiltration was obvious and edema increased in the model group,indicating successful modeling;inflammatory cell infiltration increased in the 24 rolling treatment group,and myofiber fracture and necrosis were more serious,and inflammation improved significantly in the 7 d rolling treatment group with the best effect,and the difference with the normal group was small.The results of ELISA showed that compared with the same time blank group,the TNF-α and IL-1 β contents were significantly increased in the model group(P＜0.01);compared with the same time model group,the expression contents were increased in the 24 h rolling treatment group,and decreased in all groups at the remaining time points,and significantly decreased in the 7 d rolling treatment group compared with the model group(P＜0.05).Western Blot results showed that compared with the blank group,the model group NF-κB p65 protein content was significantly increased in the model group compared with the blank group(P＜0.01);compared with the same time model group,NF-κB p65 was reduced in each rolling treatment group at the remaining time points except 24 h,with the largest ratio of decrease at 7 d(P＜0.05);the IκB α protein content in the model group decreased rapidly after modeling(P＜0.01);Compared with the same time model group,each rolling treatment group increased(P＜0.05)at the remaining time points except 24 h,and the ratio of increase was the most at 7 d(P＜0.05).Conclusion:Rolling method acting on damaged skeletal muscle can down-regulate TNF-α and IL-1 β,inhibit NF-κB signaling,reduce apoptosis of skeletal muscle injury cells,and improve the quality of functional recovery.
Skeletal muscle blunt contusionTuinaRolling methodNF-κB signal pathwayNF-κB p65IκB α
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