Sedative mechanism of Guizhi Jia Longgu Muli Decoction on the anxiety-like behaviour of the insomnia model rats with Po unrest in the lung
Objective:To examine the sedative effect and mechanism of Guizhi Jia Longgu Muli Decoction on the anxiety-like behaviour of the insomnia model rats with Po unrest in the lung from the composition of the prescription.Methods:Seventy-two rats were randomly distributed into normal group,model group,Eszopiclone group,Guizhi Jia Longgu Muli Decoction group(each of the former 4 groups has 12 rats),Guizhi Decoction group,Guizhi Jia Longgu Decoction group,Guizhi Jia Muli Decoction group,Longgu Muli group(each of the later 4 groups has 6 rats and only their whole body functions were detected).The rats except the normal group were deprived of sleep for 42 days by self-made narrow multi-platforms in the shallow water environment.From the 15th day to the 42nd day,the rats were intervened correspondingly.Then pulmonary function examination,elevated plus maze test and EEG monitoring were performed.The cingulate gyrus nerve cells were observed by nissl staining,and the GAB AAR γ 2,NMDAR,mGluR1 proteins in the brain cingulate gyrus were detected by immunohistochemistry method in the first 4 groups.Results:Compared with the normal group,pulmonary TVb,MVb,PIF,the number of open arm entries and stay time,proportion of open arm stay time,NREM,REM,average time of each sleep bont and GABAAR γ 2 decreased(P＜0.01,P＜0.05),while the respiratory frequency,the times of arousal,NMDAR and mGluRl increased in the model group(P＜0.01,P＜0.05).Nissl bodies of neurons became smaller and arranged disorderly;glial cells increased.Compared with the model group,the number of open arm entries and stay time,proportion of open arm stay time,REM,NREM,average time of each sleep bout,GABAAR γ 2 protein increased significantly(P＜0.01,P＜0.05),respiratory frequency,the times of arousal,the expression of NMDAR and mGluR1 protein decreased significantly(P＜0.01)in Eszopiclone group and Guizhi Jia Longgu Muli Decoction group with more nissl bodies.Compared with the Eszopiclone group,open arm residence time and ratio,REM,mGluRl protein expression were significantly increased in the Chinese medicine group(P＜0.01),nissl bodies of neurons increased and arranged orderly,glial cells decreased.Compared with model group,there were some statistical differences in the indexes of respiratory monitor and high plus maze test in the Guizhi Decoction group,Guizhi Jia Longgu Decoction group,Guizhi Jia Muli Decoction group,Longgu Muli group,but their effects were weaker than that of the Guizhi Jia Longgu Muli group.Conclusion:The curative effect of Guizhi Jia Longgu Muli Decoction on the insomnia rats with unrest Po in the lung does exist and is related to the synergism yin-yang and ying-wei regulation of Guizhi Decoction and Po sedation of Longgu Muli.
Guizhi Jia Longgu Muli DecoctionPo unrest in the lungInsomniaRapid eye movement(REM)Sedative effectMechanism
NETL
NSTL
万方数据
