Mechanism of Tongxie Yaofang on gastric ulcer with liver-depression and spleen-deficiency syndrome based on metabolomics
Objective:To investigate the effects of Tongxie Yaofang(TXYF)on fecal metabolism and the potential mechanism of action in rats with liver-depression and spleen-deficiency syndrome of gastric ulcer using non-targeted metabolomics.Methods:SD rats were randomly divided into blank control group,model group and TXYF group,with 6 rats in each group,and the drugs were administered by gavage.A multifactorial composite simulation of Chinese medicine etiology combined with acetic acid cauterization was used to replicate the rat model of gastric ulcer with liver depression and spleen deficiency syndrome.By histopathologic detection of gastric sinus,serum pathological and biochemical index levels,Western Blot(WB)experiments,ultra-high performance liquid chromatography(UPLC)-quadrupole-time-of-flight mass spectrometry(UPLC-TOF-MS),etc.,combined with the Human Metabolome Data Bank(HMDB)and the Genome Data Bank(KEGG)with MetaboAnalyst 5.0,to analyze the corresponding metabolic pathways of the biomarkers and the pathway enrichment and expression levels of related proteins.Results:TXYF was able to reduce the serum levels of NE and 5-HT(P＜0.01),and increase the serum levels of GAS and D-xylose(P＜0.01),and significantly decreased the protein expression of p-PI3K/PI3K(P＜0.01),and up-regulated the protein expression of p-Akt1/Akt1(P＜0.01),and at the same time,TXYF was able to alleviate the pathological damage caused by the gastric mucosa of the rat gastric ulcer in the syndrome of liver-depression and spleen-deficiency.A total of 19 potential biomarkers with the most significant differences were screened,involving major differential metabolic pathways such as amino acid metabolism,energy metabolism and lipid metabolism.Conclusion:TXYF can reduce gastrointestinal inflammatory reaction,alleviate the pathological process of gastric ulcer model rats with liver-depression and spleen-deficiency,and promote the repair of gastric mucosal tissues by regulating amino acid metabolism,energy metabolism and lipid metabolism,and inhibiting inflammatory reaction and oxidative stress.
Tongxie YaofangLiver-depressing and spleen-deficiency syndromeGastric ulcerNon-targeted metabolomicsAmino acid metabolism
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