Metabolomics study of GC-MS-based Aurantii Fructus water decoction inhibition of THP-1-derived macrophage foaming
Objective:To study the metabolomics and possible mechanism of the water detection of Aurantii Fructus(AFD)inhibiting the foaming of THP-1-derived macrophages induced by ox-LDL.Methods:THP-1 cell was divided into control group,model group,low-dose,middle-dose and high-dose groups.Oil red O staining was used to detect the formation of foam cells,ELISA was used to detect the levels of intracellular triglyceride(TG),total cholesterol(TC)and inflammatory factors[monocyte chemoattractant protein-1(MCP-1),interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α)],and GC-MS was used to detect the cell metabolome in each group to find the differential metabolites related to drug efficacy,and predict related metabolic pathways.Results:AFD could inhibit foam cell formation and reduce the expression levels of TG,TC,MCP-1,IFN-γ,TNF-α(P＜0.01,P＜0.05).Metabolomics analysis showed that 7 biomarkers including L-serine,L-threonine,and L-methionine were closely related to cell foaming,and were significantly reversed after administration(P＜0.05,P＜0.01).Conclusion:AFD can inhibit cell foaming of macrophages by regulating glycine/serine metabolism,sphingolipid metabolism and methionine metabolism,and provide a basis for the treatment of atherosclerosis.
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