HPLC同时测定白疕三黄软膏中大黄素和大黄酚的含量
Simultaneous determination of emodin and chrysophanol in Baibi Sanhuang Ointment by HPLC
宋玮 1雷晴 1王楷 1周水涵 1余倩颖 1米雄飞 1陈明岭 1肖敏1
作者信息
- 1. 成都中医药大学附属医院,成都 610072
- 折叠
摘要
目的:建立高效液相色谱(HPLC)同时测定白疕三黄软膏中大黄素和大黄酚含量的方法.方法:采用中谱红RD-C18色谱柱(4.6 mm×250 mm,5 μm),以甲醇-0.1％磷酸溶液(80:20)为流动相进行等度洗脱,检测波长为254 nm,柱温为30℃,流速为1.0mL/min.结果:大黄素、大黄酚分离度良好,其线性范围分别为0.19～6.04 μ g/mL(r=0.9999)、1.00～31.88 μ g/mL(r=1.0000);其精密度、重复性及稳定性的 RSD≤1.86％;平均回收率为96.8～103.6(RSD≤3.7％);3批白疕三黄软膏中大黄素和大黄酚的平均含量分别为16.78～22.51、117.19～131.65 μg/mL.结论:本研究所建方法准确,重复性良好,可快速测定白疕三黄软膏中大黄素和大黄酚的含量,为其质量控制提供参考.
Abstract
Objective:To develop a high performance liquid chromatography(HPLC)method for simultaneous determination of emodin and chrysophanol in Baibi Sanhuang Ointment.Methods:Isocratic elution was performed on a RD-C18 column(4.6 mm×250 mm,5 μm)with methanol-0.1％phosphoric acid solution(80:20)as mobile phase,the detection wavelength was set at 254 nm,the column temperature was 30 ℃,and the flow rate was 1.0 mUmin.Results:The separation degree of emodin and chrysophanol were good,their linear range were 0.19-6.04 μg/mL(r=0.9999),1.00-31.88 μg/mL(r=1.0000),respectively.The RSDs of precision,repeatability,and stability were ≤1.86％;and the average recovery were in the range of 96.8-103.6(RSD≤3.7％);The average content ranges of emodin and chrysophanol in 3 batches of Baibi Sanhuang Ointment were 16.78-22.51 and 117.19-131.65 μg/mL,respectively.Conclusion:The method developed in this study was accurate,and the repeatability was good,which can quickly achieve determination of emodin and chrysophanol in Baibi Sanhuang Ointment,and to provide reference for the quality control of Baibi Sanhuang Ointment.
关键词
白疕三黄软膏/高效液相色谱法/含量测定/大黄素/大黄酚Key words
Baibi Sanhuang Ointment/High performance liquid chromatography(HPLC)/Content determination/Emodin/Chrysophanol引用本文复制引用
基金项目
国家中医药局全国名中医工作室建设项目(第二届)(国中医药人教发[2022]5号)
中医药发展补助资金-省名中医工作室项目(500004003)
成都中医药大学"杏林学者"学科人才科研提升计划(QJRC2021007)
出版年
2024
NETL
NSTL
万方数据