首页|藏药普尔那黄酮纯化工艺及抗氧化活性研究

藏药普尔那黄酮纯化工艺及抗氧化活性研究

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目的:以藏药普尔那为原料,开展其黄酮纯化工艺及抗氧化活性研究.方法:采用静态吸附与解吸试验,考察8种不同型号大孔树脂对普尔那黄酮吸附-解吸性能,筛选出最佳大孔树脂型号,通过对工艺各参数优化,确定黄酮最佳纯化工艺,以体外自由基清除实验、还原力实验和基于斑马鱼模型的体内抗氧化活性为考察指标,比较纯化前后普尔那黄酮抗氧化能力的差异.结果:普尔那黄酮最佳纯化工艺为:采用DA-201-CIV型大孔树脂,在上样液浓度为1.2 mg/mL,上样速度为2 BV/h,上样体积为3 BV,洗脱溶剂为70％乙醇溶液,洗脱速度为2BV/h的条件下,收集0.7～2 BV洗脱范围内洗脱液,可使普尔那黄酮含量达到(38.60±0.57)％.抗氧化试验结果表明,普尔那黄酮具有较强的清除自由基能力以及良好的还原力,纯化后的普尔那黄酮在一定浓度范围内可抑制由甲硝唑诱导的斑马鱼皮肤荧光细胞氧化损伤.结论:通过大孔树脂纯化,可使普尔那黄酮纯度提升1.84倍,基于体外抗氧化试验及斑马鱼模型,明确了普尔那黄酮的抗氧化能力,其可用于抗氧化剂产品的开发.
Purification technology and antioxidant activity of Tibetan medicine Artemisia vestita Wall.ex Bess flavonoids
Objective:To study the purification process and antioxidant activity of flavonoids from Tibetan medicine Artemisia vestita Wall.ex Bess.Methods:Static adsorption test was used to investigate the adsorption and desorption performance of 8 different types of macroporous resin for flavonoids of Artemisia vestita Wall.ex Bess,and the optimal macroporous resin model was selected.The optimal purification process of flavonoids was determined by optimizing the process parameters.In vitro free radical scavenging assay,reducing power assay and antioxidant activity in vivo based on zebrafish model were used to compare the difference of antioxidant capacity of Artemisia vestita Wall.ex Bess flavonoids before and after purification.Results:The best purification process of Artemisia vestita Wall.ex Bess flavonoids was:DA-201-CIV macroporous resin was used to collect the eluent in the range of 0.7～2 BV under the conditions of loading solution concentration of 1.2 mg/mL,loading speed of 2 BV/h,loading volume of 3 BV,eluting solvent of 70％ethanol,and elution speed of 2 BV/h.The content of Artemisia vestita Wall.ex Bess flavonoids could reach(38.60±0.57)％.Antioxidant test results showed that Artemisia vestita Wall.ex Bess flavonoids had strong scavenging ability and good reducing power,and purified Artemisia vestita Wall.ex Bess flavonoids could inhibit the oxidative damage of skin fluorescence cells induced by metronidazole in a certain concentration range.Conclusion:Purified by macroporous resin,the purity of Artemisia vestita Wall.ex Bess flavonoids is increased by 1.84 times.Based on in vitro antioxidant test and zebrafish model,the antioxidant capacity of Artemisia vestita Wall.ex Bess flavonoids is determined,which could be used in the development of antioxidant products.

Tibetan medicine Artemisia vestita Wall.ex BessFlavonoidsMacroporous resinZebrafishAntioxidant activity

邱钺姿、徐凤华、陈静、夏青、张云、刘可春、林厚文、张姗姗、李晓彬

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齐鲁工业大学(山东省科学院),山东省科学院生物研究所,济南 250103

山东省人类疾病斑马鱼模型与药物筛选工程技术研究中心,济南 250103

青岛大学基础医学院,青岛 266000

西藏藏医药大学,拉萨 850000

上海交通大学医学院附属仁济医院,上海 200127

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藏药普尔那 黄酮 大孔树脂 斑马鱼 抗氧化活性

国家自然科学基金青年科学基金西藏藏医药大学中医学(藏医)博士点建设项目(2020)西藏藏医药大学中医学(藏医)博士点建设项目(2020)山东省重点研发计划(重大科技创新工程)济南市高等学校20条资助项目藏医药区域协同创新中心项目山东省"泰山学者"特聘专家项目

42006090BSDJS-20-04BSDJS-20-182021CXGC0105072020GXRC0312019XTCX011ts20190950

2024

中华中医药杂志
中华中医药学会

中华中医药杂志

CSTPCD北大核心
影响因子：1.135
ISSN：1673-1727
年,卷(期)：2024.39(4)
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