Construction and evaluation of animal model of central fatigue with syndrome of liver depression and spleen deficiency
Objective:Using a modified multi-platform water environment combined with dietary disorder induction method,we established a stable animal model and evaluation system that mimics the syndrome of central fatigue and liver depression combined with spleen deficiency.Methods:Thirty SPF male Wistar rats were randomly allocated into a blank control group and a model group.The model group was induced using a method of standing on a small platform in water combined with fasting every other day,while no intervention measures were applied to the blank control group.General observations,syndrome scale evaluations,behavioral assessments,HE pathological staining of duodenum,stomach,whole brain,and quadriceps muscle tissues,as well as measurements of serum AST,ALT,BUN,LAC,and LDH levels,were carried out.ELISA was used to analyze serum levels of ALD,ADH,VIP,ATP,and ADP,along with hepatic and muscular glycogen content in the liver and muscle tissues.Results:Compared with the blank control group,the model group displayed signs of growth stagnation,mental sluggishness,and decreased activity.After modeling,the model group exhibited reduced grip strength,shortened time to exhaustion,decreased upright time,grooming time,percentage of entries into the central area,percentage of time spent in the central area in the open field test,decreased percentage of entries and time spent in the open arms in the elevated plus maze test,decreased struggling and increased immobility time in the tail suspension test.In the Morris water maze test,the escape latency increased,while the number of platform crossings,time spent in the target quadrant,and distance traveled in the target quadrant all decreased.Serum levels of ALT,AST,BUN,LAC,LDH,ALD,ADH,VIP,and ADP increased,while hepatic glycogen,muscular glycogen,and ATP decreased,and fecal water content increased.Pathological changes or abnormalities were observed in the duodenum,stomach,CA1 region of the hippocampus,and quadriceps muscle of the rats in the model group.Conclusion:The animal model constructed in this study closely resembles the characteristics of central fatigue,liver depression,and spleen deficiency syndrome observed clinically,providing a new platform for the development of novel drugs and mechanistic research in the future.
Central fatigueSyndrome of liver depression and spleen deficiencyAnimal modelCombination of disease and syndromeRat
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